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Molecular Imaging and Biology

, Volume 21, Issue 2, pp 228–231 | Cite as

Imaging Pulmonary Foreign Body Reaction Using [125I]iodo-DPA-713 SPECT/CT in Mice

  • Julian Sanchez-Bautista
  • Catherine A. Foss
  • Alvaro A. Ordonez
  • Mariah H. Klunk
  • Sanjay K. JainEmail author
Brief Article
  • 97 Downloads

Abstract

Purpose

Foreign body reactions elicit granulomatous inflammation composed of reactive macrophages. We hypothesized that [125I]iodo-DPA-713 single-photon emission computed tomography (SPECT), a low-molecular-weight pyrazolopyrimidine ligand selectively trapped by phagocytes, could be used to detect foreign body reactions in a murine model.

Procedures

C57BL/6 mice intratracheally inoculated with dextran beads, which developed foreign body lesions, were imaged after injection of [125I]iodo-DPA-713 or DPA-713-IRDye800CW using SPECT and optical imaging, respectively.

Results

Foreign body lesions were clearly observed in the lungs of the dextran-treated mice on computer tomography imaging and demonstrated significantly higher [125I]iodo-DPA-713 uptake compared with control animals (p < 0.01). Ex vivo studies demonstrated granulomatous reactions in the lungs of dextran-treated mice and localization of DPA-713-IRDye800CW at the diseased sites confirming the imaging findings.

Conclusion

Radioiodinated DPA-713 may be used as a noninvasive biomarker for the detection of pulmonary foreign body reactions.

Key words

Foreign body Iodo-DPA-713 Granulomatous inflammation 

Notes

Funding

This study was funded by the National Institutes of Health (NIH) Director’s Transformative Research Award R01-EB020539 (S.K.J.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11307_2018_1249_MOESM1_ESM.pdf (244 kb)
ESM 1 (PDF 243 kb)

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Copyright information

© World Molecular Imaging Society 2018

Authors and Affiliations

  • Julian Sanchez-Bautista
    • 1
    • 2
    • 3
  • Catherine A. Foss
    • 1
    • 4
  • Alvaro A. Ordonez
    • 1
    • 2
    • 3
  • Mariah H. Klunk
    • 1
    • 2
    • 3
  • Sanjay K. Jain
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Center for Infection and Inflammation Imaging ResearchJohns Hopkins UniversityBaltimoreUSA
  2. 2.Center for Tuberculosis ResearchJohns Hopkins UniversityBaltimoreUSA
  3. 3.Department of PediatricsJohns Hopkins UniversityBaltimoreUSA
  4. 4.Russell H. Morgan Department of Radiology and Radiological SciencesJohns Hopkins UniversityBaltimoreUSA

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