Relationship between the lipid composition of maternal plasma and infant plasma through breast milk
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This study was motivated by the report that infant development correlates with particular lipids in infant plasma.
The hypothesis was that the abundance of these candidate biomarkers is influenced by the dietary intake of the infant.
A cohort of 30 exclusively-breastfeeding mother–infant pairs from a small region of West Africa was used for this observational study. Plasma and milk from the mother and plasma from her infant were collected within 24 h, 3 months post partum. The lipid, sterol and glyceride composition was surveyed using direct infusion MS in positive and negative ion modes. Analysis employed a combination of univariate and multivariate tests.
The lipid profiles of mother and infant plasma samples are similar but distinguishable, and both are distinct from milk. Phosphatidylcholines (PC), cholesteryl esters (CEs) and cholesterol were more abundant in mothers with respect to their infants, e.g. PC(34:1) was 5.66% in mothers but 3.61% in infants (p = 3.60 × 10−10), CE(18:2) was 8.05% in mothers but 5.18% in infants (p = 1.37 × 10−11) whilst TGs were lower in mothers with respect to their infants, e.g. TG(52:2) was 2.74% in mothers and 4.23% in infants (p = 1.63 × 10−05). A latent structure model showed that four lipids in infant plasma previously shown to be biomarkers clustered with cholesteryl esters in the maternal circulation.
This study found evidence that the abundance of individual lipid isoforms associated with infant development are associated with the abundance of individual molecular species in the mother’s circulation.
KeywordsCandidate biomarkers Lipids Glycerides Infant development Breastfeeding
Fatty acid residue
Latent structure model
Odd-chain-containing fatty acid residue
PC plasmalogen (sometimes known as alkyl-PCs)
World Health Organisation
Breastfeeding is generally regarded as the best start to a baby’s life. Babies who are breastfed typically have a healthy growth trajectory, suggesting that human milk provides the infant with the optimum diet for its development. Babies who are formula-fed can exhibit a weaker growth pattern and, when the process is managed poorly, can become malnourished (David and David 1984; Nisar et al. 2016). These and other considerations have led the WHO to advise that all children are breastfed exclusively for the first 6 months post partum (WHO 2002, 2009).
The differences observed in the growth trajectories of breast- and formula-fed babies may have a molecular origin. Recent evidence has shown that the circulation of breast-fed infants has a markedly different lipid and triglyceride profile compared to those fed formula milk (Koulman et al. 2014; Prentice et al. 2015). Evidence from animal models suggests that this may be due to nutritional programming. An animal model of diet-induced obesity and gestational diabetes mellitus (GDM) has shown that there is a profound effect on infants of their mother’s dietary intake and metabolism (Alfaradhi et al. 2016; Blackmore et al. 2014; Loche et al. 2018; Samuelsson et al. 2008).
This mounting body of evidence led us to the general hypothesis that there is a relationship between the molecular composition (especially lipids, glycerides and sterols) of the maternal plasma, milk and her infant. Data published to date has provided evidence that the maternal diet is reflected in the composition of the milk produced (Brenna et al. 2007; Lassek and Gaulin 2014; Martin et al. 2012; Sabel et al. 2009) consistent with the considerable compositional variety in human milk (Gibson and Kneebone 1981; Stam et al. 2013). We therefore elected to test the relationship between the lipid composition of maternal plasma and infant plasma through the milk.
Specifically, we employed the hypothesis that the molecular profile of the mothers’ plasma may not only be dependent upon the direct influx (Fig. 1) but also shaped by metabolic processes. To test these hypotheses, we used the relative abundance of the five phospholipids in infant circulation that showed an association with weight and catch-up growth in our previous study, [PC(18:1/16:0) and PC-O(34:1)] and poor weight gain [PC(20:4/18:0), PC-O(36:4) and SM(d18:1/16:0)] (Prentice et al. 2015), in 30 Gambian infants.
Lipids were detected using high resolution mass spectrometry by direct infusion in both positive and negative ionisation modes in order to profile glycerides, sterols, zwitterionic lipids and anionic lipids. The relative abundance of each species identified was calculated separately for positive and negative ionisation modes. This approach built on work on the triglyceride profile of milks (Dugo et al. 2006) and early studies (Breckenridge and Kuksis 1967; Breckenridge and Kuksis 1968), but also allowed us to profile fats, lipids and sterols in order to explore their relationships as candidate biomarkers (CBMs) of growth. Each of the development lipids was cross-checked for a correlation with any lipid in the breast milk the infant received and then checked with the lipids in the mothers’ circulation.
2.1 Study design
This study was designed to determine which species if any in infant plasma are associated with the milk they are fed, and which species if any in the milk are associated with the mothers’ plasma. This necessitated an homogeneous group of participants with a similar diet, consistent sample collection, detailed measurement of molecular abundance and statistical analyses.
Infant Z-scores were calculated using anthropometric measurements and WHO software, compared to international growth references (WHO)
Mothers (n = 30)
25.6 ± 6.4
Parity median (range)
162.8 ± 4.8
57.5 ± 6.7
21.7 ± 2.6
33.5 ± 5.9
Infants (n = 30)
13.6 ± 1.8
% Male infants
61.8 ± 4.9
5.9 ± 0.9
− 0.4 ± 0.9
0.2 ± 1.3
− 0.5 ± 1.5
2.3 Reagents and standards
Solvents were purchased from Sigma-Aldrich Ltd (Gillingham, Dorset, UK) of at least HPLC grade and were not purified further. Lipid standards were purchased from Avanti Polar lipids (Alabaster, AL; through Instruchemie, Delfzijl, NL) and used without purification. Consumables were purchased from Sarstedt AG & Co (Leicester, UK).
Mothers attended morning clinics at MRC Unit The Gambia at LSHTM Keneba situated in the Kiang West region of Gambia (hereafter referred to as MRC Keneba) with their infants, April–August 2013. The meta-data of the participants is shown in Table 1. These subjects were chosen due to a relatively homogeneous maternal diet, breastfeeding practice, ethnicity, BMI and age range. All participants were healthy.
2.5 Blood sample collection and processing
A sample of maternal plasma, milk and infant plasma were collected from each mother–infant pair (n = 30 pairs) within 24 h, and collected from all pairs between April and August 2013.
Blood was drawn by venepuncture from fasted individuals. Maternal fasted blood sampling times ranged from 09:03 to 11:45 h, and infant times ranged from 09:00 to 11:50 h. Infants were fed 0.20–2.65 h before venepuncture. Lithium Hepaparin plasma monovette tubes were placed on ice immediately and centrifuged (4 °C, 1800 × g for 20 min (HNR: Mistral 6000 Centrifuge, Sanyo Gallenkamp PLC., Leicester, UK; MRC Keneba: Centrifuge 5810R, Eppendorf, Stevenage, UK) within 1 h of collection. Centrifugation was repeated for slightly haemolysed samples. Lithium Heparin plasma aliquots were transferred to polypropylene microtubes (1.5 mL, Sarstedt AG & Co, Leicester, UK), and stored at − 80 to − 70 °C in the MRC Keneba laboratory until analysed (Keneba-SOP-4045; HNR-SOP-0367).
2.6 Milk sample collection and processing
Milk was untreated fresh hindmilk (12.5–15 mL) that was collected in the morning between 07:28 and 12:20 h, within 24 h of venepuncture, and was stored at − 80 to − 70 °C. Samples were collected at the mother’s compound and transported to MRC Keneba. Fat was measured by the creamatocrit method (50–70 μL, 75 μL glass capillary, plugged with Cristaseal) on fresh samples (Lucas et al. 1978), after which aliquots (1 mL) were transported (− 78 °C) and stored − 40 °C. Samples were thawed on ice and centrifuged (13k × g, 15 min, HNR: Heraeus PICO 17 Centrifuge, Thermo Fisher Scientific Inc., Leicestershire, UK; MRC Keneba: Haematospin 1300, Hawksley & Sons Ltd., Sussex, UK). Breast milk fat concentrations were found to be normally distributed. There were no significant differences between days in breast milk fat (ANOVA adjusted for subject ID and study day number, Scheffe’s post hoc test, p = 0.5–0.9). The overall mean ± SD (range) of the concentration of fat in breast milk was 38.3 ± 15.0 (5.9–98.4) g/L.
2.7 Extraction of the lipid fraction
The phospholipid, triglyceride and sterol fractions were isolated together using a method reported recently (Koulman et al. 2014). Briefly, plasma (25 µL) was injected, along with blank and Quality Control samples (QCs) in the wells of a glass coated 2.4 mL/well ninety-six-well plate (96w plate; Plate+™, Esslab, Hadleigh, UK). Water (100 μL, MilliQ) was added to each of the wells, followed by methanol (250 μL, HPLC grade, spiked with 0.6 μM 1,2-di-O-octadecyl–sn-glycero-3-phosphocholine, 1.2 μM 1,2-di-O-phytanyl–sn-glycero-3-phosphoethanolamine, 0.6 μM C8-ceramide, 0.6 μM N–heptadecanoyl-d-erythro-sphingosylphosporylcholine, 6.2 μM undecanoic acid, 0.6 μM trilaurin), followed by tert-butyl methyl ether (TMBE, 500 μL). The plates were then sealed (aluminium microplate sealing tape), agitated (10 min, 600 rpm) and centrifuged (10 min, 3.2k × g). A 96-channel pipette was used to transfer 200 μL of the organic solution to a glass-coated 240 μL 96w plate (Plate+™, Esslab, Hadleigh, UK). The plate was transferred to a Genevac EZ-2 evaporator (Genevac Ltd., Ipswich, UK) and dried. The samples were reconstituted (tertiary-butylmethyl ether, 25 μL and MS-mix [7.5 mM ammonium acetate in IPA:CH3OH (2:1)], 90 μL) and transferred to a glass-coated 384w plate and sealed immediately.
2.8 Mass spectrometry
Samples were then direct-infused into an Exactive Orbitrap (Thermo, Hemel Hampstead, UK), using a Triversa Nanomate (Advion, Ithaca US). Samples were ionised at 1.2 kV. The Exactive started acquiring data 20 s after sample aspiration began. After 72 s of acquisition in positive mode the Nanomate and the Exactive switched to negative mode, decreasing the voltage to − 1.5 kV. The spray was maintained for another 66 s, after which the analysis was stopped and the tip discarded, before the analysis of the next sample began. Throughout the analysis the sample plate was kept at 10 °C. Samples were run in row order.
The phospholipid, sterol and glyceride signals obtained were used to calculate relative abundance of that variable (and were therefore ‘semi-quantitative’ signals) with the signal intensity of each variable expressed relative to the total lipid signal intensity after subtraction of blank samples and removal of signals that were greater than 8 ppm different from the expected (monoisotopic) mass. Separate calculations were made for signals acquired in the two modes. Some variable comprised two or more isobars (same m/z). Raw high-resolution mass-spectrometry data were processed using XCMS (www.bioconductor.org) and Peakpicker v 2.0 (an in-house R script). Only species that were measured in more than 5% of samples were included in further analyses. 1248 lipid signals were detected robustly using this method. The lipids have been identified as described previously (Acharjee et al. 2017; Furse and Koulman 2019; Koulman et al. 2014; Prentice et al. 2015) and the identification is at level 2 of the Metabolomics Standards Initiative.
2.9 Statistical methods
Multivariate calculations were performed using Metabolanalyst 3.0 or 4.0 (Chong et al. 2018), univariate and bivariate comparisons were performed using Microsoft Excel 2013.
Univariate calculations were typically based on mean and standard deviation (±) or for the bivariate calculations a Student’s T-test was employed. Where relevant, significance thresholds were corrected for multiple variables p-value thresholds were based on the variables being dependent. Thus, thresholds were calculated based on 0.05 divided by the square root of the number of variables (1248 variables), giving a FDR threshold p value of 0.0014. In addition to Pearson correlation and Principal Component Analysis, a Latent Structure Model (LSM) clustering was employed that used the Bayesian Hierarchical Clustering (BHC) algorithm, a highly successful non-parametric multinomial Dirichlet Process (infinite mixture) model clustering tool (Heller and Ghahramani 2005; Savage et al. 2009) available as R/BHC in Bioconductor [available at https://www.bioconductor.org/packages/release/bioc/manuals/BHC/man/BHC.pdf].
Lower abundance species found in the positive mode include phosphatidylethanolamine (PE), ceramide and cholesterol (Fig. 3b). Profiling of the anionic lipids (taken in negative ion mode) suggested that PS was the most abundant (net) anionic lipid, with PI about a third as abundant (Table S4).
Both mammary gland activity such as de novo lipogenesis and the digestion have a considerable effect on the profile of lipids and triglycerides in the process and probably only a small proportion of lipids will pass from the mother’s circulation to the infant’s circulation unaffected. This led us to examine how the CBMs for both healthy growth and poor growth in infant plasma may be connected to the flow of lipids and fats from the mothers’ circulation to the infants’ (Fig. 1). The composition of milk is notably different to either maternal or infant plasma, however it is undoubtedly the only dietary influencer of infant plasma lipids in exclusively breastfed infants.
Pearson correlation coefficients of candidate biomarkers for infant development (top left), previously identified in infant plasma, with molecular species in the breast milk they are fed (left to right) and the correlations of the species identified in breast milk with molecular species in maternal plasma (lower right)
These data show that there is a relationship between the CBMs in infant plasma and several lipids and triglycerides in the milk consumed. As the lipids and fats in the maternal blood plasma supply the mammary glands for milk production, we developed the hypothesis that there would be a relationship between lipid profile of the breastfeeding mother’s blood plasma and the milk produced. Our data suggest a considerable number and broad range of lipids in the maternal plasma that may have a relationship with the milk lipid identified as being associated with infant growth (Table 2).
These data indicate that in this cohort of native Gambian women, all biomarkers appear but there is an association between four of the five, i.e. PC(34:1), PC-O(34:1), PC(38:4) and PC-O(36:4), SM(34:2). The data also imply that the species in milk with the strongest relationships with these candidate biomarkers and molecular species in the maternal circulation are TG(49:4), (55:2), (55:3), (55:5), SM(45:0) and SM(47:1) as they are identified by both methods. This is remarkable because it in indicates that odd-chain-containing FAs must be present in the milk of women whose diet contains very little dairy or other source of OCFARs.
The results in this paper detail the lipid profile of plasma samples from infants and their mothers, and fresh samples of the milk that passed between them within 24 h of collection of the plasma. The samples were taken from a cohort of 30 pairs between April and August 2013, a period that covered the end of the dry season and the beginning of the wet season, from a group of women with a roughly similar diet.
The abundance of lipid classes in milk (Tables S3 and S4) is reasonably consistent with previous work on the lipid and glyceride composition of this fluid (Bitman and Wood 1990; Bitman et al. 1983; Rodríguez-Alcalá and Fontecha 2010). However, the relatively high abundance of DGs, the reduced abundance of TGs, the normal abundance of SMs but low abundance of PCs suggests that the DG fraction is a composite of several sources, probably including lipases that are active in the milk (Bengtsson-Olivecrona and Olivecrona 1991; Deeth 2006; Neville et al. 1991) and during lipid collection (Furse et al. 2015a; Furse and Killian 2013), but mainly as an artefact of ionisation in mass spectrometry.
The proportions of phospholipids in the positive and negative ionisation modes are hard to compare due to poor cross-over and different ionisation efficiencies across the range and between modes. However a study of the phospholipid composition of bovine and ovine milk using phosphorus NMR (Murgia et al. 2003) showed that the ratio of PS to PI was ~ 1:9 and ~ 1:2.1, respectively. The proportions of PC, PE and SM were roughly the same in Cows’ milk, with PC around 80% of the abundance of SM and PE in Ewes’ milk. These data suggest that human milk is similar to these two, but with some distinct characteristics, and that negative ionisation mode is more similar to 31P NMR than to positive ionisation mode.
The Pearson coefficients calculated in the present analysis (Table 2) show that there are significant correlations between the CBMs for good and poor infant growth and certain TGs in the human milk, and between these TGs in the human milk and the profile of the mothers’ plasma. It is remarkable that a number of the TGs comprising 43–57 carbons, which is only possible through the presence of fatty acid residues with an odd number of carbon atoms (OCFARs), have emerged as CBMs. This suggests the incorporation of either or both C15 and C17 (margaric acid) FAs. Dairy foods can supply C15, however there is not a clear dietary source for C17, suggesting that it is produced endogenously. This is consistent with recent work indicates that the C15 found in humans has a dietary origin, where C17 is produced endogenously (Jenkins et al. 2017). As the supply of this results from the activity of the enzyme of the Hacl1 gene in humans, the question of whether there is a link between a reduction in the abundance of stearic acid and the supply of TGs in milk that are associated with infant development is raised. Further work using labelled analogues may offer insight into the precise role of these species in vivo.
Evidence for a role of the mother’s diet and health in the metabolic health of infants is accumulating (Aaltonen et al. 2010; Duque-Guimarães and Ozanne 2013; Fernandez-Twinn and Ozanne 2010; Linderborg et al. 2014) [review (Lönnerdal 1986)], and that of fathers is just beginning to emerge (Watkins et al. 2018). Specifically, the fatty acid residues (FARs) in the maternal milk are driven by FAs in the diet and the exchange of fats between the circulation and stores in the adipose and hepatic tissue, amongst other processes (Brenna et al. 2007; Lassek and Gaulin 2014; Martin et al. 2012; Sabel et al. 2009). On the other hand, myristic acid residues (14:0) are relatively abundant in the milk, which cannot come from the circulation. The evidence presented here supports the conclusion that there is extensive remodelling of the lipid and glyceride profile in the mammary glands, and again during infant digestion.
Recent work on the abundance individual lipid and glyceride species in human plasma has begun with direct infusion mass spectrometry in positive ionisation mode, detecting PCs, SMs, TGs, cholesterol and CEs (Koulman et al. 2014; Prentice et al. 2015). This work has shown that the abundance of several lipids changes as infants reach 12 months, and again after 12 months. Some isoforms of CE and TG only appear after 1 year, as do several isoforms of SM and PC (Koulman et al. 2014). Several commonplace isoforms also differ in abundance between formula- and breast-fed infants (Prentice et al. 2015). This suggests that the lipid profile of infants in circulo is affected by a number of exposures.
An unsupervised LSM (Fig. 5) was used as an orthogonal method to Pearson correlations in order to explore whether the same relationships between variables were observed in the global molecular profile. As this approach indicates that four of the five candidate biomarkers are clustered together (Cluster 7), and that several of the odd-chain-containing triglycerides cluster together (Clusters 11 and 12). We suggest that this is suitable evidence for further investigation of the role of these species in vivo.
Although we present the different sample types sequentially, based on the assertion that the plasma composition affects the milk composition and that in turn affects the circulating lipids of the infant, it cannot be excluded that all three lipid pools are also driven by other physiological processes that are responsible for these correlations. This is most likely for the correlations found between variables in the maternal plasma and milk as both samples are drawn from one individual. It is however relevant to determine the causes of the correlations observed as they offer a way of changing the lipid composition of the milk in a way that would affect the growth and development of the infant. This is important for situations where breastfeeding is not yielding a healthy growth trajectory. More generally, these data support the conclusion that there is a relationship between the lipid profiles at the various stages that shapes or even governs the supply of individual nutrients to infants, and thence the infant’s development.
The authors would like to thank Dr Matt Castle for helpful discussions about statistical methods. Funding from the BBSRC for SF and AK (Grant Nos. BB/M027252/1 and BB/M027252/2), SGS and AK (BB/P028195/1) and AK (NIHR 146281) is gratefully acknowledged.
SF conceived and designed the research, conducted research, analysed data and prepared and revised the manuscript. GB conceived the research, collected and managed the collection of samples and data. SGS designed the test using the LSM, analysed data and revised the manuscript. JS analysed data and revised the manuscript. GG conceived the research and acquired funding for it and supervised GB. AK conceived and designed the research, conducted research, analysed samples, collected data, supervised the project and revised the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent was obtained from all individual participants included in the study.
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