Metabolic diversity in tuber tissues of native Chiloé potatoes and commercial cultivars of Solanum tuberosum ssp. tuberosum L.
The native potatoes (Solanum tuberosum ssp. tuberosum L.) cultivated on Chiloé Island in southern Chile have great variability in terms of tuber shape, size, color and flavor. These traits have been preserved throughout generations due to the geographical position of Chiloé, as well as the different uses given by local farmers.
The present study aimed to investigate the diversity of metabolites in skin and pulp tissues of eleven native accessions of potatoes from Chile, and evaluate the metabolite associations between tuber tissues.
For a deeper characterization of these accessions, we performed a comprehensive metabolic study in skin and pulp tissues of tubers, 3 months after harvesting. Specific targeted quantification of metabolites using 96 well microplates, and high-performance liquid chromatography combined with non-targeted metabolite profiling by gas chromatography time-of-flight mass spectrometry were used in this study.
We observed differential levels of antioxidant activity and phenolic compounds between skin and pulp compared to a common commercial cultivar (Desireé). In addition, we uncovered considerable metabolite variability between different tuber tissues and between native potatoes. Network correlation analysis revealed different metabolite associations among tuber tissues that indicate distinct associations between primary metabolite and anthocyanin levels, and antioxidant activity in skin and pulp tissues. Moreover, multivariate analysis lead to the grouping of native and commercial cultivars based on metabolites from both skin and pulp tissues.
As well as providing important information to potato producers and breeding programs on the levels of health relevant phytochemicals and other abundant metabolites such as starch, proteins and amino acids, this study highlights the associations of different metabolites in tuber skins and pulp, indicating the need for distinct strategies for metabolic engineering in these tissues. Furthermore, this study shows that native Chilean potato accessions have great potential as a natural source of phytochemicals.
KeywordsGenetic variation Native potatoes Exotic germplasms Metabolite profiling
This work was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [grant number CBB - AUC-00018-16]. Research fellowships were granted by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to ANN and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [Grant Number CBB-BPD- 00019-16] to FMOS. ANN, MRD and CIB also acknowledge the support from the Chilean Ministerio de Educación (MEC-CONICYT; Grant PAI80160036). The authors wish to thank the NUBIOMOL-UFV for providing the facilities for the analysis of this work, and Michael Handford (Universidad de Chile) for language support.
CIB, MRD, and ANN conceived and designed the research. FD and CIB performed the experiments. TO, MM and FMdeOS contributed powerful analytical tools. CIB, FMdeOS and MM analyzed data. CIB and ANN wrote the manuscript. MRD, TO, JS and ARF revised the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects.
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