Purinergic Signalling

, Volume 15, Issue 3, pp 357–366 | Cite as

Multiple adenosine receptor subtypes stimulate wound healing in human EA.hy926 endothelial cells

  • Zeinab Bonyanian
  • Matthew Walker
  • Eugene Du Toit
  • Roselyn B. Rose’MeyerEmail author
Original Article


Wound healing is an important outcome of tissue damage and can be stimulated by adenosine released from cells during events such as tissue injury, ischaemia or tumour growth. The aim of this research was to determine the potency and efficacy of adenosine A1, A2A and A2B receptor agonists on the rate of wound healing and cell proliferation in human EA.hy926 endothelial cells. Real-time PCR data showed that only adenosine A1, A2A and A2B receptor mRNA were expressed in this cell line. All three adenosine receptor agonists, CPA, CGS21680 and NECA, significantly increased the rate of wound healing in human EAhy926 endothelial cells with the following order of potency CGS21680>CPA>NECA and efficacy CPA>NECA>CGS21680. The selective adenosine A1, A2A and A2B receptor antagonists, DPCPX, ZM241385 and MRS1754 (all at 10 nM), reversed the effects of their respective agonists. EAhy926 endothelial cell proliferation was also significantly increased with the adenosine A1 and A2B receptor agonists, CPA and NECA. Western blot analysis demonstrated that adenosine A2A and A1 receptor protein levels were highly expressed compared with the adenosine A2B receptors in the EAhy926 endothelial cell lines. While all three adenosine A1, A2A and A2B receptor subtypes contribute to cell proliferation and wound healing in human EAhy926 endothelial cells, treatments selectively targeting receptor subtypes may further enhance wound healing.


Adenosine Endothelial cells Wound healing Cell proliferation 



The EA.hy926 endothelial cell line used for these studies was generously donated to our research group by the Apoptosis Research Group, Griffith University, Gold Coast campus.

Compliance with ethical standards

Conflicts of interest

Zeinab Bonyanian declares that he has no conflict of interest.

Matthew Walker declares that he has no conflict of interest.

Eugene Du Toit declares that he has no conflict of interest.

Roselyn B. Rose’Meyer declares that she has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11302_2019_9668_MOESM1_ESM.docx (200 kb)
ESM 1 (DOCX 198 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Zeinab Bonyanian
    • 1
  • Matthew Walker
    • 1
  • Eugene Du Toit
    • 1
  • Roselyn B. Rose’Meyer
    • 1
    Email author
  1. 1.School of Medical SciencesGriffith UniversityQueenslandAustralia

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