Light-induced ATP release from the lens
The recent discovery of the photoreceptor melanopsin in lens epithelial cells has opened the possibility of modulating this protein by light stimulation. Experiments carried out on New Zealand white rabbits have demonstrated that the release of ATP from the lens to the aqueous humor can be reduced either when a yellow filter or a melanopsin antagonist is used. Compared to control (1.10 ± 0.15 μM ATP), the application of a yellow filter (λ465–480) reduced ATP in the aqueous humor 70%, while the melanopsin antagonist AA92593 reduced the presence of ATP 63% (n = 5), an effect which was also obtained with the PLC inhibitor U73122. These results indicate that when melanopsin is blocked either by the lack of light, a filter, or an antagonist, the extracellular presence of ATP is significantly reduced. This discovery may be relevant, on the one hand, because many ocular physiological processes are controlled by ATP and, on the other hand, because it is possible to stimulate ATP release with just light and without using any added substance.
KeywordsAA92593 ATP Eye Lens Light Melanopsin
This work was supported by research grants from the Spanish Ministry of Economy and Competitivity (SAF-2013-44416-R, SAF2016-77084R) and the Ministry of Health Social Services and Equality RETICS (Grant RETICS RD 16/0008/0017 and RD12/0034/0001).
Compliance with ethical standards
Conflicts of interest
Jesús Pintor declares that he has no conflict of interest.
This study followed the ARVO Statement for the Use of Animals in Opthalmic and Vision Research and the European Communities Council Directive (86/609/EEC).
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