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Tree Genetics & Genomes

, 15:84 | Cite as

Hierarchical genetic and spatial structure among varieties and populations of Hymenaea stigonocarpa (Fabaceae) in Brazilian savannah

  • Ramilla dos Santos Braga
  • Rafael Barbosa Pinto
  • Lázaro José Chaves
  • José Alexandre Felizola Diniz-Filho
  • Thannya Nascimento Soares
  • Rosane Garcia Collevatti
  • Mariana Pires de Campos TellesEmail author
Original Article
  • 27 Downloads
Part of the following topical collections:
  1. Population structure

Abstract

Approaches in population genetics decompose species genetic variation at hierarchical level, understanding the action of microevolutionary processes at spatial scales. We evaluated the taxonomically complex species Hymenaea stigonocarpa, a tree species distributed in central Brazil, presenting three botanical varieties weakly distinguished by morphological traits. We aimed to investigate the spatial genetic structure of populations of H. stigonocarpa along Cerrado biome, assessing genetic differentiation and the existence of hybridization zones in this species. We genotyped 901 plants from 28 local populations using nine nuclear microsatellite loci. Analyses of genetic diversity, Bayesian inference estimated genetic differentiation and migration rate among populations and varieties. Mantel tests and sPCA evaluated spatial pattern of genetic variation. We observed that H. stigonocarpa var. brevipetiolata presented larger genetic differentiation compared to H. stigonocarpa var. stigonocarpa and H. stigonocarpa var. pubescens (FCT = 0.175). Bayesian approaches supported a genetic different cluster and hybridization process in sympatric populations for H. stigonocarpa var. brevipetiolata. Migration rate was high and not significantly higher among varieties, confirming the existence of hybridization. Spatial correlations were relatively low, but with exponential decrease of genetic similarity along of the geographic space. Spatial genetic structure was higher for H. stigonocarpa var. brevipetiolata and local structures were found with sPCA, indicating that neighboring populations are genetically different mainly in populations of eastern Cerrado. The genetic clusters are not congruent with the vegetative characters used to recognize the three botanical varieties, which should be viewed with skepticism. Our genetic analyses strongly support the need for a taxonomic review.

Keywords

Brazilian Cerrado Genetic differentiation Genetic resource Hierarchical AMOVA jatobá-do-cerrado Microsatellite 

Notes

Acknowledgments

Our research has been continuously supported by several grants and fellowships of the research network GENPAC (Geographical Genetics and Regional Planning for Natural Resources in Brazilian Cerrado) from CNPq/FAPEG (projects #563839/2010-4 and #201110267000125), by the CERGEN (Núcleo de Excelência em Recursos Genéticos Vegetais do Cerrado -PRONEX/FAPEG/CNPq 07-2012), PRONEM FAPEG -  Process 201710267000539 and by the CNPq/Universal project 447754/2014-9. We thank Systema Naturae for Environmental Consultant for helping with field work. Our study has also been developed in the context of the National Institutes for Science and Technology in Ecology, Evolution and Biodiversity Conservation (INCT_EECBio), supported by MCTIC/CNPq (process #465610/2014-5) and FAPEG, in addition to support from PPGS CAPES/FAPEG (Public Call #08/2014). R.S. Braga was supported by fellowships from CAPES. L.J. Chaves, T.N. Soares, R.G. Collevatti, and M.P.C. Telles were supported by productivity grants from CNPq.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Data archiving statement

Data will be deposited in the Dryad repository (http://datadryad.org/) after acceptance for review.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ramilla dos Santos Braga
    • 1
  • Rafael Barbosa Pinto
    • 1
  • Lázaro José Chaves
    • 2
  • José Alexandre Felizola Diniz-Filho
    • 3
  • Thannya Nascimento Soares
    • 1
  • Rosane Garcia Collevatti
    • 1
  • Mariana Pires de Campos Telles
    • 1
    • 4
    Email author
  1. 1.Laboratório de Genética & Biodiversidade, Instituto de Ciências BiológicasUniversidade Federal de GoiásGoiâniaBrazil
  2. 2.Escola de AgronomiaUniversidade Federal de GoiásGoiâniaBrazil
  3. 3.Departamento de Ecologia, Instituto de Ciências BiológicasUniversidade Federal de CP 131GoiâniaBrazil
  4. 4.Escola de Ciências Agrárias e BiológicasPontifícia Universidade Católica de GoiásGoiâniaBrazil

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