Tree Genetics & Genomes

, 14:81 | Cite as

Expressional characterization of galacturonosyltransferase-like gene family in Eucalyptus grandis implies a role in abiotic stress responses

  • Longjun ChengEmail author
  • Xiaoxiang Ni
  • Mi Zheng
  • Lijuan Sun
  • Xiaofei Wang
  • Zaikang Tong
Original Article
Part of the following topical collections:
  1. Gene Expression


Glycosyltransferase (GT) plays a pivotal role in cell wall biosynthesis in plants. Galacturonosyltransferase-like (GATL) genes, belonging to the GT8 family, have been proven to be involved in pectin and/or xylan biosynthesis of the cell wall. Here, we identified eight GATL genes from the Eucalyptus grandis genome and characterized the gene structure and chromosomal location. The genes were found to be distributed across five chromosomes, including two pairs in block duplication regions. None of the EgrGATL genes contained introns. And, with the exception of EgrGATL8, the remainder of the EgrGATL proteins possessed the three classic motifs characteristic of all GATL proteins. Expression analysis in the different tissues showed that EgrGATL1, EgrGATL4, and EgrGATL8 were highly expressed in xylem and phloem; EgrGATL6 exhibited the highest expression in leaves, and in phloem and leaves, EgrGATL2 and EgrGATL3 both exhibited very low expression. However, the abiotic stress response of plants can be affected by changes in the components and structure of the cell wall. The expression patterns of EgrGALTs under low-temperature, high-temperature, drought, salinity, and abscisic acid (ABA) treatments were assessed by qRT-PCR. The results showed that most of the EgrGATL genes could be induced under low temperature, and some were even able to increase their expression level under high temperature. Under drought conditions, the expression levels of most of the genes initially increased and then decreased. Similar expression patterns were observed in leaves under treatment with NaCl and ABA. Our results provide fundamental information towards the functional dissection of EgrGATL genes and their potential involvement in improving plant abiotic stress tolerance.


Eucalyptus grandis Galacturonosyltransferase-like Gene expression Abiotic stress ABA 



This work was supported by the State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) grant 201101, Zhejiang Province Key Project for Science and Technology grant (2016C02056). We thank the anonymous reviewers for their comments and suggestions on the manuscript.

Data archiving statement

The annotated nucleotide sequences of GATL genes in E. grandis, A. thaliana, O. sativa, and P. trichocarpa in NCBI GenBank database are in the following accession numbers: XM_010030945, XM_010045536, XM_010027746, XM_010045995, XM_010039834, XM_010072152, XM_010071957, and XM_001988622; NM_101787, NM_113753, NM_114936, NM_101196, NM_111501, NM_100152, NM_116131, NM_102263, NM_105677, and NM_178954; XM_015786450, XM_015767508, XM_015777620, XM_015781767, XM_015790219, XM_015777291, and XM_015773839; and XM_002302433, XM_024595933, XM_006383195, XM_002310744, XM_002312345, XM_002311900, XM_002311753, XM_024609252, XM_002314848, XM_002315384, XM_006374993, and XM_002320288. Details of the sequences are listed in Table S3 in supplementary data files.

Supplementary material

11295_2018_1294_MOESM1_ESM.xlsx (16 kb)
ESM 1 (XLSX 16 kb)
11295_2018_1294_MOESM2_ESM.doc (322 kb)
ESM 2 (DOC 321 kb)


  1. Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, Ren J, Li WW, Noble WS (2009) MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res 37:W202–W208CrossRefPubMedPubMedCentralGoogle Scholar
  2. Baldwin L, Domon JM, Klimek JF, Fournet F, Sellier H, Gillet F, Pelloux J, Lejeune-Hénaut I, Carpita NC, Rayon C (2014) Structural alteration of cell wall pectins accompanies pea development in response to cold. Phytochemistry 104:37–47CrossRefPubMedGoogle Scholar
  3. Bo H, Jin J, Guo AY, He Z et al (2015) GSDS 2.0: an upgraded gene feature visualization server. Bioinformatics 31:1296–1297CrossRefGoogle Scholar
  4. Breton C, Šnajdrová L, Jeanneau C et al (2006) Structures and mechanisms of glycosyltransferases. Glycobiology 16:29–37CrossRefGoogle Scholar
  5. Caffall KH, Pattathil S, Phillips SE, Hahn MG, Mohnen D (2009) Arabidopsis thaliana T-DNA mutants implicate GAUT genes in the biosynthesis of pectin and xylan in cell walls and seed testa. Mol Plant 2:1000–1014CrossRefPubMedGoogle Scholar
  6. Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3:1–30CrossRefPubMedGoogle Scholar
  7. Chatterjee M, Berbezy P, Vyas D, Coates S, Barsby T (2005) Reduced expression of a protein homologous to glycogenin leads to reduction of starch content in Arabidopsis leaves. Plant Sci 168:501–509CrossRefGoogle Scholar
  8. Domon JM, Baldwin L, Acket S, Caudeville E, Arnoult S, Zub H, Gillet F, Lejeune-Hénaut I, Brancourt-Hulmel M, Pelloux J, Rayon C (2013) Cell wall compositional modifications of Miscanthus ecotypes in response to cold acclimation. Phytochemistry 85:51–61CrossRefPubMedGoogle Scholar
  9. Fujita Y, Fujita M, Shinozaki K, Yamaguchi-Shinozaki K (2011) ABA-mediated transcriptional regulation in response to osmotic stress in plants. J Plant Res 124:509–525CrossRefPubMedGoogle Scholar
  10. de Godoy F, Bermúdez L, Lira BS et al (2013) Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato. J Exp Bot 64:2449–2466CrossRefPubMedPubMedCentralGoogle Scholar
  11. Gomord V, Wee E, Faye L (1999) Protein retention and localization in the endoplasmic reticulum and the Golgi apparatus. Biochimie 81:607–618CrossRefPubMedGoogle Scholar
  12. Han X, Yin H, Song X, Zhang Y, Liu M, Sang J, jiang J, Li J, Zhuo R (2016) Integration of small RNAs, degradome and transcriptome sequencing in hyperaccumulator Sedum alfredii uncovers a complex regulatory network and provides insights into cadmium phytoremediation. Plant Biotechnol J 14:1470–1483CrossRefPubMedPubMedCentralGoogle Scholar
  13. Harfouche A, Meilan R, Altman A (2014) Molecular and physiological responses to abiotic stress in forest trees and their relevance to tree improvement. Tree Physiol 34:1181–1198CrossRefPubMedGoogle Scholar
  14. Höfte H, Peaucelle A, Braybrook S (2012) Cell wall mechanics and growth control in plants: the role of pectins revisited. Front Plant Sci 3:121PubMedPubMedCentralGoogle Scholar
  15. Houston K, Tucker MR, Chowdhury J et al (2016) The plant cell wall: a complex and dynamic structure as revealed by the responses of genes under stress conditions. Front Plant Sci 7:984CrossRefPubMedPubMedCentralGoogle Scholar
  16. Keegstra K, Raikhel N (2001) Plant glycosyltransferases. Curr Opin Plant Biol 4:219–224CrossRefPubMedGoogle Scholar
  17. Kirch R, Astarita LV, Santarém ER et al (2011) Eucalyptus transgenic plants: from genetic transformation protocols to biosafety analysis. BMC Proc 5(suppl 7):179CrossRefGoogle Scholar
  18. Kong Y, Zhou G, Avci U, Gu X, Jones C, Yin Y, Xu Y, Hahn MG (2009) Two poplar glycosyltransferase genes, PdGATL1. 1 and PdGATL1. 2, are functional orthologs to PARVUS/AtGATL1 in Arabidopsis. Mol Plant 2:1040–1050CrossRefPubMedGoogle Scholar
  19. Kong Y, Zhou G, Yin Y, Xu Y, Pattathil S, Hahn MG (2011) Molecular analysis of a family of Arabidopsis genes related to galacturonosyltransferases. Plant Physiol 155:1791–1805CrossRefPubMedPubMedCentralGoogle Scholar
  20. Kong Y, Zhou G, Abdeen AA, Schafhauser J, Richardson B, Atmodjo MA, Jung J, Wicker L, Mohnen D, Western T, Hahn MG (2013) GALACTURONOSYLTRANSFERASE-LIKE5 is involved in the production of Arabidopsis seed coat mucilage. Plant Physiol 163:1203–1217CrossRefPubMedPubMedCentralGoogle Scholar
  21. Kubacka-Zębalska M, Kacperska A (1999) Low temperature-induced modifications of cell wall content and polysaccharide composition in leaves of winter oilseed rape (Brassica napus L. var. oleifera L.). Plant Sci 148:59–67CrossRefGoogle Scholar
  22. Le Gall H, Philippe F, Domon JM et al (2015) Cell wall metabolism in response to abiotic stress. Plants 4:112–166CrossRefPubMedPubMedCentralGoogle Scholar
  23. Lee C, Zhong R, Richardson EA, Himmelsbach DS, McPhail BT, Ye ZH (2007) The PARVUS gene is expressed in cells undergoing secondary wall thickening and is essential for glucuronoxylan biosynthesis. Plant Cell Physiol 48:1659–1672CrossRefPubMedGoogle Scholar
  24. Lescot M, Déhais P, Thijs G et al (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325–327CrossRefPubMedPubMedCentralGoogle Scholar
  25. Leucci MR, Lenucci MS, Piro G, Dalessandro G (2008) Water stress and cell wall polysaccharides in the apical root zone of wheat cultivars varying in drought tolerance. J Plant Physiol 165:1168–1180CrossRefPubMedGoogle Scholar
  26. Liu J, Luo M, Yan X, Yu C, Li S (2016) Characterization of genes coding for galacturonosyltransferase-like (GATL) proteins in rice. Genes Genom 38:917–929CrossRefGoogle Scholar
  27. Martinez JP, Silva H, Ledent JF et al (2007) Effect of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaseolus vulgaris L.). Eur J Agron 26:30–38CrossRefGoogle Scholar
  28. Mehrotra R, Bhalothia P, Bansal P, Basantani MK, Bharti V, Mehrotra S (2014) Abscisic acid and abiotic stress tolerance-different tiers of regulation. J Plant Physiol 171:486–496CrossRefPubMedGoogle Scholar
  29. Pilling E, Höfte H (2003) Feedback from the wall. Curr Opin Plant Biol 6:611–616CrossRefPubMedGoogle Scholar
  30. Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Report 15:8–15CrossRefGoogle Scholar
  31. R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria ISBN 3-900051-07-0, URL Google Scholar
  32. Sarkar D (2008) Lattice: multivariate data visualization with R partI. Springer Science & Business Media, New York, p 55–87Google Scholar
  33. Scheible WR, Pauly M (2004) Glycosyltransferases and cell wall biosynthesis: novel players and insights. Curr Opin Plant Biol 7:285–295CrossRefPubMedGoogle Scholar
  34. Seifert GJ, Blaukopf C (2010) Irritable walls: the plant extracellular matrix and signaling. Plant Physiol 153:467–478CrossRefPubMedPubMedCentralGoogle Scholar
  35. Sterling JD, Atmodjo MA, Inwood SE, Kumar Kolli VS, Quigley HF, Hahn MG, Mohnen D (2006) Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase. PNAS 103:5236–5241CrossRefPubMedGoogle Scholar
  36. Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K (2002) Important roles of drought-and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant J 29:417–426CrossRefPubMedGoogle Scholar
  37. Tenhaken R (2015) Cell wall remodeling under abiotic stress. Front Plant Sci 5:1–9CrossRefGoogle Scholar
  38. Ulvskov P (2011) Annual plant reviews, Plant polysaccharides: biosynthesis and bioengineering. John Wiley & Sons 41:93–212Google Scholar
  39. Van Bel M, Diels T, Vancaester E et al (2017) PLAZA 4.0: an integrative resource for functional, evolutionary and comparative plant genomics. Nucleic Acids Res 46(D1):D1190–D1196PubMedCentralGoogle Scholar
  40. Yin Y, Chen H, Hahn MG, Mohnen D, Xu Y (2010) Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8. Plant Physiol 153:1729–1746CrossRefPubMedPubMedCentralGoogle Scholar
  41. Yoshida T, Mogami J, Yamaguchi-Shinozaki K (2014) ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Curr Opin Plant Biol 21:133–139CrossRefPubMedGoogle Scholar
  42. Zhu JK (2016) Abiotic stress signaling and responses in plants. Cell 167:313–324CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Longjun Cheng
    • 1
    Email author
  • Xiaoxiang Ni
    • 1
  • Mi Zheng
    • 2
  • Lijuan Sun
    • 1
  • Xiaofei Wang
    • 1
  • Zaikang Tong
    • 1
  1. 1.State Key Laboratory of Subtropical Silviculture, College of Forestry and BiotechnologyZhejiang Agriculture and Forestry UniversityHangzhouChina
  2. 2.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinChina

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