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Tree Genetics & Genomes

, 14:69 | Cite as

Genomic structure, QTL mapping, and molecular markers of lipase genes responsible for palm oil acidity in the oil palm (Elaeis guineensis Jacq.)

  • Hubert Domonhédo
  • Teresa Cuéllar
  • Sandra Espeout
  • Gaëtan Droc
  • Marilyne Summo
  • Ronan Rivallan
  • David Cros
  • Bruno Nouy
  • Alphonse Omoré
  • Léifi Nodichao
  • Vincent Arondel
  • Corneille Ahanhanzo
  • Norbert Billotte
Original Article
  • 139 Downloads
Part of the following topical collections:
  1. Complex Traits

Abstract

The degradation of triglycerides in oil palm fruit due to an endogenous lipase in the pulp is the main reason for acidification of palm oil, which causes major economic losses and is currently mainly associated with the FLL1 gene. We designed this study to identify all the major genes controlling differences in acidity and lipase activity in the oil palm fruit mesocarp and determine a molecular markers kit to allow marker-assisted selection of commercial varieties with low acidity. Not only one gene (FLL1) but three closely linked genes including FLL1 were found and characterized in LM2T_EgCIR184O12c, a bacterial artificial chromosome sequence of 231 kb. Intra-gene PCR-based markers were designed for these genes. A QTL gene co-localization analysis for oil acidity (percentage of fatty acids released) was performed on two mapping populations. It evidenced a single major QTL at our lipase gene loci, explaining 84 to 92% of phenotypic variation, and validating the main genetic control of palm oil acidification by FLL1 and/or by the two new lipase genes. The three lipase genes had high homology to demonstrated triacylglycerol lipases. While FLL1 shows the highest expression levels, the two other genes may also contribute to oil acidity. Our molecular markers of lipase genes and the associated major QTL is an important step towards marker-assisted selection of commercial varieties with low acidity.

Keywords

E. guineensis Palm oil acidity Lipase QTL FLL1 

Notes

Acknowledgments

Hubert Domonhedo is a recipient of the French Embassy SCAC scholarship and the West Africa Agricultural Productivity Program (WAAPP) fellowship. The authors are grateful to the CRA-PP of INRAB for providing the plant samples and phenotypic data. We would also like to thank the Ivory Coast’s Centre National de Recherche Agronomique (CNRA) for providing the LM2T oil palm leaf material previously used to construct the oil palm BAC library. We would furthermore like to express our gratitude to the reviewers of this journal for their corrections and kind help in improving this article. Finally, we are grateful to Dr. Fabienne Morcillo (IRD, France) for providing the PCR primers for the probes of candidate gene FLL1.

Funding

This study was funded by the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), PalmElit S.A.S., and the Centre de Recherches Agricoles Plantes Pérennes (CRA-PP) of the Institut National des Recherches Agricoles du Bénin (INRAB).

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hubert Domonhédo
    • 1
    • 2
  • Teresa Cuéllar
    • 3
    • 4
  • Sandra Espeout
    • 3
    • 4
  • Gaëtan Droc
    • 3
    • 4
  • Marilyne Summo
    • 3
    • 4
  • Ronan Rivallan
    • 3
    • 4
  • David Cros
    • 3
    • 4
  • Bruno Nouy
    • 5
  • Alphonse Omoré
    • 1
  • Léifi Nodichao
    • 1
  • Vincent Arondel
    • 6
  • Corneille Ahanhanzo
    • 6
  • Norbert Billotte
    • 3
    • 4
  1. 1.Centre de Recherches Agricoles-Plantes Pérennes (CRA-PP). Institut National des Recherches Agricoles du Bénin (INRAB)BP 1 PobèBénin
  2. 2.Laboratoire Central des Biotechnologies Végétales et d’Amélioration des Plantes; Faculté des Sciences et Techniques (FAST)Université d’Abomey-Calavi (UAC)CotonouBénin
  3. 3.Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Bios-UMR AGAPMontpellier Cedex 5France
  4. 4.CIRAD, INRA, Montpellier SupAgroUniversity of MontpellierMontpellierFrance
  5. 5.PalmElit SASMontferrier sur LezFrance
  6. 6.University of BordeauxVillenave d’OrnonFrance

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