Quantitative trait loci (QTL) mapping of blush skin and flowering time in a European pear (Pyrus communis) progeny of ‘Flamingo’ × ‘Abate Fetel’
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Blush skin and flowering time are agronomic traits of interest to the Agricultural Research Council (ARC) Infruitec-Nietvoorbij pear breeding programme. The genetic control of these traits was investigated in the pear progeny derived from ‘Flamingo’ (blush cultivar) × ‘Abate Fetel’ (slightly blush) made up of 121 seedlings. Blush skin was scored phenotypically over three seasons and flowering time was scored over two seasons. A total of 160 loci from 137 simple sequence repeat (SSR) markers were scored in the progeny and used to construct parental genetic linkage maps. Quantitative trait loci (QTL) analysis revealed two QTLs for blush skin, a major QTL on linkage group (LG) 5 in ‘Flamingo’, and a major QTL on LG9 in ‘Abate Fetel’. Two SSR markers, NB101a and SAmsCO865954, were closely linked with the major QTL on LG5 in ‘Flamingo’, with alleles 139 bp and 462 bp in coupling, respectively. These markers were present in approximately 90% of the seedlings scored as good blush (class 4) based on the average data set. These two markers were used to genotype other pear accessions to validate the QTL on LG5 with the view of marker-assisted selection. Two candidate genes, MYB86 and UDP-glucosyl transferase, were associated with the QTL on LG5 and MYB21 and MYB39 were associated with the QTL on LG9. QTL analysis for flowering time revealed a major QTL located on LG9 in both parents. Marker GD142 with allele 161 bp from ‘Flamingo’ was present in approximately 88% of the seedlings that flowered earlier than either parent, based on the average data set. The QTLs and linked markers will facilitate marker-assisted selection for the improvement of these complex traits.
KeywordsEuropean pear Blush skin Flowering time Candidate genes SSRs QTLs
SM Ntladi thanks the National Research Foundation (NRF) for a Professional Development Programme (PDP) PhD studentship. Other costs were funded by the NRF Technology and Human Resource for Industry Programme (THRIP) (grants TP2011062500004 and TP1407249871) and the Agricultural Research Council (ARC). Mardé Booyse is thanked for statistical analysis of the phenotypic data. Justin Lashbrooke and Fabrizio Costa are thanked for analysis of anthocyanin-related genes. Kashief Soeker is acknowledged for screening of the associated markers in 25 pear accessions.
Data archiving statement
PCR multiplexes made up of pear and apple SSR primer pairs used to genotype the ‘Flamingo’ × ‘Abate Fetel’ pear progeny for mapping are provided in Table S1.
Blush skin data sets used to detect QTLs in the ‘Flamingo’ × ‘Abate Fetel’ progeny are provided in Table S2.
Flowering time data sets (Julian date of 80% full bloom) used to detect QTLs in the ‘Flamingo’ × ‘Abate Fetel’ progeny are provided in Table S3.
Information on markers not used for mapping in the progeny of ‘Flamingo’ × ‘Abate Fetel’ is provided in Table S4.
Genotypic data for the SSR markers in the pear progeny of ‘Flamingo’ × ‘Abate Fetel’ are provided in Table S5.
Position of the QTLs for blush skin detected on LG5 in ‘Flamingo’ and LG9 in ‘Abate Fetel’ in the pear progeny of ‘Flamingo’ × ‘Abate Fetel’ based on grouping of classes is provided in Fig. S1.
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