Abstract
Objective
To compare the effects of β-cryptoxanthin (β-cry) from citrus fruit on bone-formation parameters in the distal femoral epiphysis of ovariectomized (OVX) mice with the effects of osteoporosis therapeutic agents.
Methods
Ten-week-old OVX ICR mice were used. The mice were divided into five experimental groups: β-cry; bisphosphonate (treatment with the bone resorption inhibitor risedronate); vitamin K2 [treatment with the bone formation stimulator and bone resorption inhibitor menaquinone 4 (MK4)]; OVX control; and sham (no OVX). Risedronate was used as the bisphosphonate (risedronate group), and menaquinone 4 was used as vitamin K2 (MK4 group). Risedronate was administered orally, and β-cry and MK4 were mixed with the regular feed. The mice were fed the diets for 2 months. Femurs were resected, and blood samples were collected. Bone mineral density (BMD) was analyzed by peripheral quantitative computed tomography. Microcomputed tomography was also performed to evaluate the three-dimensional structure of the trabecular bone.
Results
In contrast to the risedronate and MK4 groups, there was no improvement in the trabecular BMD, cortical thickness, or periosteal perimeter in the β-cry group. Trabecular structural parameters including the morphometric indices, continuity index, and connectivity index were significantly improved in the β-cry group compared with the OVX control group. In particular, there was a marked improvement in morphometric indices.
Conclusion
β-Cry improved the trabecular structure. This suggests that β-cry might be useful as a dietary supplement to prevent bone degeneration and osteoporosis associated with aging.
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Iino, M., Kozai, Y., Kawamata, R. et al. Effects of β-cryptoxanthin on bone-formation parameters in the distal femoral epiphysis of ovariectomized mice. Oral Radiol 30, 1–8 (2014). https://doi.org/10.1007/s11282-013-0131-7
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DOI: https://doi.org/10.1007/s11282-013-0131-7