Dynamics of nitrogen transformation and bacterial community with different aeration depths in malodorous river

  • Jinghan Chen
  • Yan HeEmail author
  • Jianhua Wang
  • Minsheng Huang
  • Cuixiang Guo
Original Paper


In this research, the dynamics of nitrogen transformation and bacterial community in malodorous river were investigated with different aeration depths. Computational flow dynamics (CFD) and Reynolds number (Re) were specially used to characterize the hydrodynamics condition under different aeration depths. The results indicated that aeration depth had vital impact on nitrogen transformation and bacterial community structure. It was found that a range of aeration depth (0.20–0.45 m above sediment–water interface) facilitated the removal of NH4+-N and TN with Re ranging between 6211 and 8930. Proteobacteria took over Firmicutes to become the predominant phylum (36–78%) under aeration, and the main subdivisions of γ-, β- and δ-Proteobacteria also varied greatly with different aeration depths. Interestingly, there was a marked shift of the inferentially identified dominant functional role within Proteobacteria from organic-matter degradation to nitrogen metabolism and then to sulfur metabolism as well as the coupling of nitrogen and sulfur with the increase of disturbance. The redundancy analysis (RDA) further confirmed the importance of aeration disturbance in shaping bacterial community. These findings help to gain improved understanding of endogenous N-behavior and aquatic microbial ecology, and underline the need for integrating the hydrodynamics factors with microbial community.

Graphic abstract


Aeration depth Bacteria Computational flow dynamics Dissolved oxygen Malodorous river sediments Nitrogen 



This work was supported by National Natural Science Foundation of China (41877477), Shanghai Natural Science Foundation (16ZR1408800), Shanghai Pujiang Talent Program (16PJD023), Shanghai Science and Technology Development Funds (18DZ1203806), and the Research Funds of The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control (1701K005).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11274_2019_2773_MOESM1_ESM.docx (22 kb)
Supplementary file1 (DOCX 23 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Jinghan Chen
    • 1
  • Yan He
    • 1
    Email author
  • Jianhua Wang
    • 1
  • Minsheng Huang
    • 1
  • Cuixiang Guo
    • 2
  1. 1.Shanghai Key Laboratory of Urbanization and Ecological Restoration, School of Ecological and Environmental Sciences, Institute of Eco-ChongmingEast China Normal UniversityShanghaiChina
  2. 2.The Guangxi Key Laboratory of Theory and Technology for Environmental Pollution ControlGuilinChina

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