Biotreatment of restaurant wastewater with an oily high concentration by newly isolated bacteria from oily sludge

  • Li-Li Gao
  • Yong-Chang Lu
  • Ji-Long Zhang
  • Jing Li
  • Jian-Dong ZhangEmail author
Original Paper


High concentration restaurant oily wastewater from restaurants and food processing industries discharged into water environment usually results in environment pollution and inhibits the activity of microorganisms in biological wastewater treatment systems. In this study, 75 strains from oily sludge were isolated with oil degradation activity for edible oil-contained wastewater. Eight isolates were able to grow well in liquid cultures with edible oil as the sole carbon source and discovered with high efficient oil-degrading ability. Seven out of eight isolates were identified as Acinetobacter and one isolate as Kluyvera cryocrescens, based on their 16S rRNA gene sequences. Three highly efficient oil degrading bacteria (Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b) were selected and their degradation characteristic were examined, the results showed that the three isolates were effective under pH range from 7.0 to 10.0, and temperature from 25 to 35 °C. For degradation of 2–4% (v/v) of vegetable oil, > 85% degradation percentage were obtained within 30 h. Degradation of the higher concentration oil (6–8%, v/v) result in 50–70% degradation percentage within 72 h, and the degradation percentage for the isolated strains were decreased about 50% for the degradation of 10% oil (< 45%) compared to 2% oil. Different type of oils were also tested, > 90% of degradation percentage were obtained by the three isolates, implied that these strains are capable of removing various oils efficiently. These results suggested that Acinetobacter dijkshoorniae LYC46-2, Kluyvera cryocrescens LYC50-1a and Acinetobacter pittii LYC73-4b are potential species could be efficiently used for high concentration restaurant oily wastewater treatment and might be applicable to a wastewater treatment system for the removal of oil.


Biotreatment Emulsification activity Oily sludge Restaurant oily wastewater Bacteria 



This study was financially supported by the Key Research and Development (R&D) Projects of Shanxi Province (201703D32111270 and 201703D321009-4), Talent Training Project of Shanxi Postgraduate Joint Training Base (2018JD17) and Shanxi Scholarship Council of China (No. 2015–042).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Research involving with human participants

This study does not describe any experimental work related to human.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Li-Li Gao
    • 1
  • Yong-Chang Lu
    • 1
  • Ji-Long Zhang
    • 2
  • Jing Li
    • 3
  • Jian-Dong Zhang
    • 3
    Email author
  1. 1.College of Environmental Science and EngineeringTaiyuan University of TechnologyTaiyuanPeople’s Republic of China
  2. 2.College of Mining EngineeringTaiyuan University of TechnologyTaiyuanPeople’s Republic of China
  3. 3.Department of Biological and Pharmaceutical Engineering, College of Biomedical EngineeringTaiyuan University of TechnologyTaiyuanChina

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