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Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism

  • Ping YuEmail author
  • Xinxin Wang
  • Qian Ren
  • Xingxing Huang
  • Tingting Yan
Original Paper
  • 72 Downloads

Abstract

Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism were investigated. The fused strain B. subtilis FS105 with the highest activity of alkaline pectinase was obtained after two rounds of genome shuffling. The activity of alkaline pectinase in B. subtilis FS105 was 499 U/ml, which was improved by 1.6 times compared to that in original strain. To elucidate its molecular mechanism, rpsL gene sequences from original and fused strains were cloned and aligned, and the space structure of their coding proteins were also analyzed and compared. The alignment of the rpsL gene sequences indicated that three bases G, G and C were respectively replaced by A, A and G in the positions 52, 408 and 409 after genome shuffling. This resulted in the substitution of two amino acid residues in ribosomal protein S12: D18N and P137A, and therefore improving the biosynthesis of alkaline pectinase. This study lays a foundation for improving the activity of alkaline pectinase by genome shuffling and understanding its molecular mechanism.

Keywords

Bacillus subtilis FS105 Alkaline pectinase Genome shuffling rpsL gene Ribosomal protein S12 

Notes

Acknowledgements

This study was supported by the Food Science and Engineering - the Most Important Discipline of Zhejiang Province, China (No. 2017SIAR214).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by the author.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ping Yu
    • 1
    Email author
  • Xinxin Wang
    • 1
  • Qian Ren
    • 1
  • Xingxing Huang
    • 1
  • Tingting Yan
    • 1
  1. 1.College of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhouPeople’s Republic of China

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