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Pseudomonas sp. UW4 acdS gene promotes primordium initiation and fruiting body development of Agaricus bisporus

  • Chaohui Zhang
  • Guang Zhang
  • Yamei Wen
  • Tao Li
  • Yuqian Gao
  • Fanmei Meng
  • Liyou QiuEmail author
  • Yuncan AiEmail author
Original Paper

Abstract

To simplify industrial mushroom cultivation, we introduced a bacterial Pseudomonas sp. UW4 acdS gene, encoding 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (AcdS), into fungus Agaricus bisporus. Transformant A. bisporus-acdS14 cased with sterilized-vermiculite generated primordia 5 days sooner than wild-type strain, confirming the specific role of the AcdS enzyme. Being consistent with the AcdS enzyme activity increased by 84%, the mycelium growth rate was increased by 25%; but, the ACC and ethylene concentrations were reduced by 71% and 36%, respectively, in the A. bisporus-acdS14 transformant. And the bacterium P. sp. UW4 attachment on the mycelium of the A. bisporus-acdS14 transformant was drastically reduced. We conclude that the heterogeneously expressed bacterial acdS gene degrades ACC and reduces ethylene-synthesis, eliminating ethylene inhibition on the mycelium growth and primordium formation in A. bisporus. Our results provide new insights into the mechanism underlying casing soil bacterium, and help formulate a casing-less cultivation for the next-generation mushroom industry.

Keywords

ACC deaminase Agaricus bisporus Ethylene Pseudomonas sp. UW4 

Notes

Acknowledgements

This work was supported by the National Spark Program of China (Grant No. 2015GA750013).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biocontrol, School of Life SciencesSun Yat-Sen UniversityGuangzhouPeople’s Republic of China
  2. 2.Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture, College of Life SciencesHenan Agricultural UniversityHenanPeople’s Republic of China
  3. 3.College of Life Science and TechnologyHenan Institute of Science and TechnologyXinxiangPeople’s Republic of China
  4. 4.Zhoukou Academy of Agricultural SciencesZhoukouPeople’s Republic of China

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