Purification and identification of a novel antifungal protein from Bacillus subtilis XB-1

  • Jianjun Ren
  • Wenhao He
  • Chunyu Li
  • Song He
  • Dongze NiuEmail author
Original Paper


This study aimed to characterize a powerful antifungal component from bacteria. Bacillus subtilis strain XB-1, which showed maximal inhibition of Monilinia fructicola, was isolated and identified, and an antifungal protein was obtained from it. Ammonium sulfate precipitation, ion exchange chromatography, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were used to purify and identify the proteins secreted by B. subtilis XB-1. Analyses revealed that purified fraction V had the strongest antifungal effect, with the largest pathogen inhibition zone diameter of 4.15 cm after 4 days (P < 0.05). This fraction showed a single band with a molecular weight of approximately 43 kDa in SDS-PAGE. Results from SDS-PAGE and liquid chromatography electrospray ionization tandem mass spectrometry analyses demonstrated that fraction V was likely a member of the chitosanase family. These results suggest that B. subtilis XB-1 and its antifungal protein may be useful in potential biocontrol applications.

Graphic abstract


Antifungal protein Bacillus subtilis Chitosanase Monilinia fructicola 



This work was financially supported by the Science Foundation of Changzhou University (Grant No. ZMF18020316), Hebei Cixin Environmental Technology Co., Ltd, China (Grant No. 2018K0948), and Shaanxi Xintiandi Grass Industry Co., Ltd, China (Grant No. 2018K0947).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Jianjun Ren
    • 1
    • 2
  • Wenhao He
    • 2
  • Chunyu Li
    • 1
  • Song He
    • 1
    • 3
  • Dongze Niu
    • 1
    Email author
  1. 1.Institute of Urban and Rural MiningChangzhou UniversityChangzhouChina
  2. 2.College of Biological Science and EngineeringBeijing University of AgricultureBeijingChina
  3. 3.School of Petrochemical EngineeringChangzhou UniversityChangzhouChina

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