Effect of temperature on Pi54-mediated leaf blast resistance in rice

  • P. Madhusudhan
  • P. SinhaEmail author
  • L. S. Rajput
  • M. Bhattacharya
  • Taru Sharma
  • V. Bhuvaneshwari
  • Kishore Gaikwad
  • S. Gopala Krishnan
  • A. K. Singh
Original Paper


Assessment of temperature effect on plant resistance against diseases has become essential under climate change scenario as temperature rise is anticipated to modify host resistance. To determine temperature influence on resistance gene, a pair of near-isogenic rice lines differing for the Pi54 resistance gene was assessed against leaf blast. Blast resistance was determined as the extent of infection efficiency (IE) and sporulation (SP) at suboptimal (22 °C and 32 °C) and optimal temperature (27 °C) of pathogen aggressiveness. Relative resistance for IE and SP was higher at suboptimal temperature as compared to that of optimal temperature. Maximum level of resistance was at 22 °C where higher levels of expression of Pi54 and defence-regulatory transcription factor WRKY45 were also noted. At 32 °C, although some level of resistance noted, but level of Pi54 and WRKY45 expression was too low, suggesting that resistance recorded at higher temperature was due to reduced pathogen aggressiveness. At the optimal temperature for pathogen aggressiveness, comparatively lower levels of Pi54 and WRKY45 expression suggest possible temperature-induced interruption of the defence processes. The variation in resistance patterns modulated by temperature is appeared to be due to pathogen’s sensitivity to temperature that leads to varying levels of Pi54 gene activation. Quick and violent activity of the pathogen at optimal temperature came into sight for the interruption of defence process activated by Pi54 gene. Evaluation of blast resistance genes under variable temperature conditions together with weather data could be applied in screening rice genotypes for selection of resistance having resilience to temperature rise.


Leaf blast Relative resistance Rice Temperature influence 



Pathogen associated molecular pattern


Effector triggered immunity/effector triggered susceptibility

Pi 54

Resistance gene


Transcription factor


Mitogen associated protein


Relative resistance for sporulation


Relative resistance for infection efficiency


Relative resistance for latent period


Infection efficiency


Latent period




Days of inoculation


Latent period indicates time taken for sporulation in 50% of the lesions


Combined relative resistance


Rate of change of a biological or chemical system as a consequence of increasing the temperature by 10 °C


Nuclear binding site- leiucine rich repeat


mRNA for Pi54 gene


mRNA for transcription factor WRKY45 gene


Nematode resistance gene


Resistance gene


Qualitative disease resistance


Transcription factor


Rice genotype susceptible to leaf blast


Indian Council of Agricultural Research-Indian Agricultural Research Institute


Number of mRNA molecules synthesized/min or transcription initiation rate /min


Activation coefficient


Occupancy − average probability that the target site will be occupied by the transcription factor and DNA − protein complex once formed


Transcriptional delay in minute


mRNA turnover rate/min



We are very much thankful to the Head Division of Plant Pathology, Joint Directors (Academic and Research) and Director ICAR-Indian Agricultural Research Institute, New Delhi 110012 and NICRA project for financial support.


Funding was provideed by Indian Council of Agricultural Research [Grant No. 14-215].


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • P. Madhusudhan
    • 1
    • 3
  • P. Sinha
    • 1
    Email author
  • L. S. Rajput
    • 1
    • 6
  • M. Bhattacharya
    • 2
  • Taru Sharma
    • 1
  • V. Bhuvaneshwari
    • 7
  • Kishore Gaikwad
    • 4
  • S. Gopala Krishnan
    • 5
  • A. K. Singh
    • 5
  1. 1.Division of Plant PathologyICAR-Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.Department of AgronomyIOWA State UniversityAmesUSA
  3. 3.Agricultural Research StationAcharya N G Ranga Agricultural UniversityNelloreIndia
  4. 4.National Institute for Plant BiotechnologyNew DelhiIndia
  5. 5.Division of GeneticsICAR-Indian Agricultural Research InstituteNew DelhiIndia
  6. 6.Division of Plant ProtectionICAR-Indian Institute of Soybean ResearchIndoreIndia
  7. 7.Regional Agricultural Research StationAcharya N G Ranga Agricultural UniversityMaruteruIndia

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