Valinomycin, produced by Streptomyces sp. S8, a key antifungal metabolite in large patch disease suppressiveness
Large patch disease, caused by Rhizoctonia solani AG2-2, is the most devastating disease in Zoysiagrass (Zoysia japonica). Current large patch disease control strategies rely primarily upon the use of chemical pesticides. Streptomyces sp. S8 is known to possess exceptional antagonistic properties that could potentially suppress the large patch pathogen found at turfgrass plantations. This study aims to demonstrate the feasibility of using the strain as a biological control mechanism. Sequencing of the S8 strain genome revealed a valinomycin biosynthesis gene cluster. This cluster is composed of the vlm1 and vlm2 genes, which are known to produce antifungal compounds. In order to verify this finding for the large patch pathogen, a valinomycin biosynthesis knockout mutant was created via the CRISPR/Cas9 system. The mutant lost antifungal activity against the large patch pathogen. Consequently, it is anticipated that eco-friendly microbial preparations derived from the S8 strain can be utilized to biologically control large patch disease.
KeywordsBiological control Large patch Streptomyces Valinomycin Zoysiagrass
This work was supported by the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Project No. 315004-5).
- Asano T, Senda M, Suga H, Kageyama K (2010) Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J Phytopathol 158:609–615Google Scholar
- Beard JB (1972) Turfgrass: science and culture. Prentice-Hall, Englewood CliffsGoogle Scholar
- Couch HB, Lucas LT, Haygood RA (1990) The nature and control of Rhizoctonia blight. Golf Course Manag 58:49–58Google Scholar
- Engelke MC, Murray JJ, Yeam DY (1983) Distribution, collection and use of zoysiagrass in the Far East, part II. Agronomy Abstract, pp. 123–125Google Scholar
- Hwang YS, Choi JS (1999) Effect of mowing interval, aeration, and fertility level on the turf quality and growth of zoysiagrass (Zoysia japonica Steud.). Kor Turfgrass Sci 13:79–90Google Scholar
- Kieser T (2000) Practical Streptomyces genetics. John Innes Foundation, NorwichGoogle Scholar
- Lee DW, Lee SM, Kim DS, Choi TH, Chang TH (2012) Application of paraffin oil for control of large patch on Zoysia japonica. Asian J Turfgrass Sci 26:17–23Google Scholar
- Song CH, Islam M, Chang TH, Lee YS (2012) Isolation and identification of antagonistic bacteria for biological control of large patch disease of zoysiagrass caused by Rhizoctonia solani AG2-2 (IV). Asian J Turfgrass Sci 26:8–16Google Scholar