Disruption of stcA blocks sterigmatocystin biosynthesis and improves echinocandin B production in Aspergillus delacroxii

  • Taoling MinEmail author
  • Lei Xiong
  • Yan Liang
  • Rui Xu
  • Chenchang Fa
  • Sheng Yang
  • Haifeng HuEmail author
Original Paper


Echinocandin B (ECB) is an important lipohexapeptide used for chemical manufacture of the antifungal agent anidulafungin. Sterigmatocystin (ST) is a polyketide mycotoxin produced by certain species of Aspergillus such as Aspergillus delacroxii SIPIW15, which could produce both ECB and ST. However, the presence of the potent carcinogen ST will greatly affect the quality and safety of ECB production. Therefore, it is essential to eliminate the ST biosynthesis and increase ECB titers in Asp. delacroxii SIPIW15. In this study, the polyketide synthase gene (stcA) required for biosynthesis of ST and its flanking region in Asp. delacroxii SIPIW15 were cloned, sequenced and analyzed firstly. Based on Agrobacterium-mediated transformation, the ΔstcA mutant AMT-1 was obtained and its yield of ECB was increased by 40% without ST detected at the same time as compared to the original strain. The results of the fed-batch experiments showed that the ECB yield of the ΔstcA strain AMT-1 was increased to 2163 ± 31 mg/l and no ST was detected in the 50 l bioreactor. This work suggested that the ΔstcA strain AMT-1 has the potential for application in ECB production improvement, and more importantly, to eliminate ST-related environmental pollution in ECB fermentation industry.

Graphic abstract


Echinocandin B Sterigmatocystin Agrobacterium-mediated transformation Aspergillus delacroxii 



This work was supported by Natural Science Foundation of Shanghai [2015ZR1440300] and Science and Technology Commission of Shanghai Municipality [11XD1423700]. We are grateful to Jun Lin and He Huang (Shanghai Institutes for Biological Sciences, Shanghai, China) for editing the English of the manuscript. We also thank Guihua Gong (Shanghai Institutes for Biological Sciences, Shanghai, China) for helpful advice in qRT-PCR.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Lab of New Drugs and Pharmaceutical Process, Shanghai Institute of Pharmaceutical IndustryChina State Institute of Pharmaceutical IndustryShanghaiChina
  2. 2.Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina

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