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Rare actinobacteria: a potential source of bioactive polyketides and peptides

  • Sunita BundaleEmail author
  • Jaya Singh
  • Deovrat Begde
  • Nandita Nashikkar
  • Avinash Upadhyay
Original Paper

Abstract

Polyketides and peptides obtained from actinobacteria are important therapeutic compounds which include front line antibiotics and anticancer drugs. Many screening programs are directed towards isolation of bioactive compounds from these organisms but the chances of finding novel antimicrobial leads among common actinobacteria are fast dwindling. As a result, the focus has shifted to the members of less exploited genera of rare actinobacteria. Three isolates, MMS8, MMS16 and KCR3 found to be potent polyketide and peptide producers were identified by 16S rRNA gene sequencing and their sequences deposited in the GenBank under the accession numbers MG407702, MG372012 and MG430204 respectively. MMS8 identified as Micromonospora auratinigra, yielded one potent compound determined to be chloroanthraquinone with an minimum inhibitory concentration (MIC) of 8 µg/ml against Bacillus subtilis and an IC50 value of 10 µg/ml and 4 μg/ml against HeLa and IMR cell lines respectively. This is the first report of the production of chloroanthraquinone by M. auratinigra. MMS16, identified as a member of the family Micromonosporaceae, yielded a potent compound MMS16B analyzed to be a novel bafilomycin analogue. The MIC of the compound was found to be 7 μg/ml against B.subtilis and IC50 value against HeLa and IMR was observed to be 9 μg/ml and 14 μg/ml respectively. MMS16B was also found to exhibit anti-quorum sensing (AQS) activity at sublethal concentrations. KCR3 identified as Kocuria kristinae yielded a novel antimicrobial peptide with antibacterial, antifungal and AQS activity. To the best of our knowledge, no antimicrobial activity has ever been reported from K. kristinae.

Keywords

Anti-quorum sensing Peptides Polyketides Rare actinobacteria 

Notes

Acknowledgements

The authors would like to acknowledge the financial assistance provided by the Department of Science and Technology, Government of India for the present study under the Scheme WOS-A. The authors would also like to thank Central Instrumentation Facility, IISER Bhopal, India for providing NMR and Mass Spectra Analysis and Material Science Department of VNIT, Nagpur, India for FT-IR analysis respectively. We acknowledge our student, Deepak Khushalani for his help in acquiring the spectral data of KCR3A.

Compliance with ethical standards

Conflict of interest:

The authors declare that they have no conflict of interest.

Supplementary material

Supplementary Fig. 1 LC–MS spectra of KCR3A i LC elution profile of the TLC purified fraction, ii MS profile of the first LC eluant (inset HRMS of the all components obtained) and iii MS profile of the last eluant. Given below are the m/z values of the components realised by LC–MS analysis

11274_2019_2668_MOESM1_ESM.jpg (85 kb)
Supplementary file1 (JPG 85 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Hislop School of BiotechnologyHislop CollegeNagpurIndia
  2. 2.Department of BiochemistryDr. Ambedkar CollegeNagpurIndia

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