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Decolorization and biodegradation of reactive Red 198 Azo dye by a new Enterococcus faecalisKlebsiella variicola bacterial consortium isolated from textile wastewater sludge

  • Hadi Eslami
  • Ahmad Shariatifar
  • Ebrahim Rafiee
  • Mahboubeh Shiranian
  • Faezeh Salehi
  • Saeede Sadat Hosseini
  • Gilda Eslami
  • Reza Ghanbari
  • Ali Asghar EbrahimiEmail author
Original Paper
  • 13 Downloads

Abstract

The present study investigated biodegradation and removal of Reactive Red 198 (RR198) dye from aqueous environments using a new bacterial consortium isolated from textile wastewater sludge on laboratory scale via batch study. Two bacterial species, Enterococcus faecalis (EF) and Klebsiella variicola (KV), were identified after isolation, through biochemical assays, Polymerase chain reaction (PCR), and 16S rRNA gene sequencing. To determine their ability to biodegrade RR198 dye, physicochemical parameters, including bacterial concentration, time, pH, and temperature, were tested; the results showed that the best conditions included a bacterial concentration of 3.5 mL × 105 cells/mL and incubation time of 72 h. Under such conditions, the removal efficiency of RR198 dye at an initial concentration of 10–25 mg/L was more than 98%; however, for concentrations of 50, 75, and 100 mg/L, removal efficiency was reduced to 55.62%, 25.82%, and 15.42%, respectively (p = 0.005). The highest removal efficiency occurred at pH 8.0, reaching 99.26% after 72 h of incubation. With increasing the incubation temperature from 25 °C to 37 °C, removal efficiency increased from 71.71 to 99.26% after 72 h of incubation, and increasing the temperature from 37 to 45 °C, the removal efficiency was reduced (p ≤ 0.001). Therefore, the EFKV bacterial consortium can be used for efficient removal of RR198 dye from textile effluent.

Keywords

Biodegradation Decolorization Rreactive red 198 Bacterial consortium Enterococcus faecalis Klebsiella variicola 

Abbreviations

RR198

Reactive red 198

EF

Enterococcus faecalis

KV

Klebsiella variicola

PCR

Polymerase chain reaction

TSB

Tryptic soy broth

SDS

Sodium dodecyl sulfate

PBS

Phosphate buffered saline

ANOVA

One-way analysis of variance

BC

Bacterial concentration

Notes

Acknowledgements

The present study is the outcome of a research project (Code: 5165) sponsored by the Environmental Science and Technology Research Center of the School of Public Health at Shahid Sadoughi University of Medical Sciences and Health Services. The authors acknowledge and thank the research center for its generous support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Ethics approval

The ethical approval conducted in Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran with code IR.SSU.SPH.REC.1395.119.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Hadi Eslami
    • 1
    • 2
  • Ahmad Shariatifar
    • 2
  • Ebrahim Rafiee
    • 2
  • Mahboubeh Shiranian
    • 2
  • Faezeh Salehi
    • 2
  • Saeede Sadat Hosseini
    • 3
  • Gilda Eslami
    • 3
  • Reza Ghanbari
    • 4
  • Ali Asghar Ebrahimi
    • 2
    Email author
  1. 1.Department of Environmental Health Engineering, School of HealthRafsanjan University of Medical SciencesRafsanjanIran
  2. 2.Environmental Science and Technology Research Center, Department of Environmental Health Engineering, School of Public HealthShahid Sadoughi University of Medical SciencesYazdIran
  3. 3.Research Center for Food Hygiene and Safety, Department of MicrobiologyShahid Sadoughi University of Medical SciencesYazdIran
  4. 4.Social Determinants of Health Research CenterQazvin University of Medical SciencesQazvinIran

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