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Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots

  • Ramesh Kumar Kushwaha
  • Sucheta Singh
  • Shiv Shanker Pandey
  • Alok Kalra
  • C. S. Vivek BabuEmail author
Original Paper

Abstract

Endophytes have been reported from all plant species from different parts of tissue including root, stem and leaves. Here we report, three fungal endophytes, Aspergillus terreus strain 2aWF (2aWF), Penicillium oxalicum strain 5aWF (5aWF), and Sarocladium kiliense strain 10aWF (10aWF) from Withania somnifera, which could enhance withanolides content in leaf and root. Upon treatment with the above endophytes to 4 weeks old plants in field conditions, W. somnifera elicited withanolide A content (97 to 100%) in leaves without considerable changes in withaferin A content. Furthermore, withanolide A content in roots of 5aWF and 10aWF endophyte treated W. somnifera plants increased up to 52% and 65% respectively. Incidentally, expression profile of withanolide and sterol biosynthetic pathway genes HMGR, DXR, FPPS, SQS, SQE, CAS, SMT1, STE1 and CYP710A1 were significantly upregulated in 2aWF, 5aWF and 10aWF fungal endophyte treated plants. Besides, modulation of withanolide biosynthetic pathway genes, fungal endophytes also induce a host resistant related gene, NPR1 resulting in 2, 4 and 16 fold expression levels in 2aWF, 10aWF and 5aWF endophyte treatments respectively, compared to control plants. Overall, our results illustrate that application of native-fungal endophytes 2aWF (96.60%), 5aWF (95%) and 10aWF (147%) enhances plant biomass in addition to withanolide content.

Keywords

Endophytes Withania somnifera Aspergillus terreus Penicillium oxalicum Sarocladium kiliense Withaferin A and Withanolide A 

Notes

Acknowledgements

This work was supported by NWP BSC0117 (XII Five Year Plan Network Project) from the Council of Scientific and Industrial Research (CSIR), India. Authors express sincere thanks to the Director, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India for his support and encouragement. RKK acknowledges Indian Council of Medical Research (ICMR), India for financial assistance in the form of fellowship and contingency grant for research activity. CSV and RKK greatly acknowledges Dr. Dinesh A Nagegowda for providing withanolides standards & primers and Dr. D.K. Venkata Rao for sharing his lab facilities.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

11274_2019_2593_MOESM1_ESM.docx (4.3 mb)
Supplementary material 1 (DOCX 4396 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Ramesh Kumar Kushwaha
    • 1
    • 3
  • Sucheta Singh
    • 2
    • 3
  • Shiv Shanker Pandey
    • 3
  • Alok Kalra
    • 2
    • 3
  • C. S. Vivek Babu
    • 1
    • 3
    Email author
  1. 1.Microbial Technology LaboratoryCSIR-Central Institute of Medicinal and Aromatic Plants, Research CentreBangaloreIndia
  2. 2.Microbial Technology DivisionCSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia
  3. 3.Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndia

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