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The phyllosphere indigenous microbiota of Brassica campestris L. change its diversity in responding to di-n-butyl phthalate pollution

  • Jiangang PanEmail author
  • Decai Jin
  • Haiming Jiang
  • Xiaoyun Leng
  • Aiai Zhang
  • Zhihui Bai
Original Paper

Abstract

In this study, the effects of di-n-butyl phthalate (DBP) on the phyllosphere bacterial community of field mustard (Brassica campestris L.) at the five-leaf stage were investigated. The indigenous alpha-diversity of the phyllosphere bacteria was altered after spraying with different concentrations of DBP. Shannon diversity indices were significantly changed on day 5 after treatment at DBP concentrations > 400 mg L−1 (P > 0.05). Nevertheless, the difference between treatment and control was not significant on day 9 after DBP treatment (P > 0.05). Exposure to DBP resulted in a decrease in Proteobacteria and Firmicutes, and an increase in Actinobacteria at all sampling intervals. These changes included significant increases in the relative abundance of Paracoccus and Rhodococcus, and significant decreases in that of Pseudomonas, Exiguobacterium, an unclassified genus of Pseudomonadaceae, and an unclassified genus of Enterobacteriaceae. This study provides new evidence for the possibility of using phyllosphere microbiota to remediate DBP contamination.

Keywords

Di-n-butyl phthalate Stress Phyllosphere Bacterial community Field mustard 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31360126), the innovation fund of Inner Mongolia University of Science & Technology for excellent youth (2017YQL08), and National Natural Science Foundation of China (No. 31560015). We would like to thank Editage [http://www.editage.cn] for English language editing. we were also grateful to Institute for Environmental Genomics, University of Oklahoma for data processing.

Supplementary material

11274_2019_2589_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 21 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of Life Science and TechnologyInner Mongolia University of Science and TechnologyBaotouChina
  2. 2.Inner Mongolia Key Laboratory for Biomass-Energy ConversionBaotouChina
  3. 3.Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina

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