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Structural characterization and applications of a novel polysaccharide produced by Azospirillum lipoferum MTCC 2306

  • Puja Kumari
  • Mrinal Bhave
  • Mukesh DobleEmail author
Original Paper
  • 159 Downloads

Abstract

Azospirillum lipoferum MTCC 2306, a free-living nitrogen fixing bacteria, has a doubling time of 1.7 h in MPSS media. At the end of 28 h at a pH of 7 and temperature of 30 °C it produces 1.8 ± 0.013 g/L biomass and 2.1 ± 0.018 g/L of cyclic beta glucan (CβG) in MPSS medium with a yield coefficient (YP/S) of 2.1. This novel polysaccharide is a water-soluble cyclic biopolymer and is generally produced by Rhizobiaceae and predominantly made up of glucose. The CβG has a degree of polymerisation varying between 10 and 13 and has both α- and β-glycosidic linkages. It is not substituted with any functional groups such as acetates or succinates. Its ability to bind to aniline blue suggests that it can be a potential candidate for being used as carrier in medical imaging as well as in reducing toxicity of textile effluents. It is able to encapsulate rifampicin, a hydrophobic drug and increase its aqueous solubility by 71%. So, CβG appears to have promising applications in the field of drug, food, cosmetic and nutraceutical industries.

Keywords

Azospirillum lipoferum Rifampicin Cyclic β glucan Dye binding Encapsulation 

Notes

Acknowledgements

The first author acknowledges the Ministry of Human Resource Development, Government of India for financial support. Department of Chemistry and Sophisticated analytical instrument facility (SAIF), IIT-Madras, for providing their analytical instrument facilities for characterization.

Supplementary material

11274_2019_2588_MOESM1_ESM.docx (770 kb)
Supplementary material 1 (DOCX 770 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biotechnology, Bhupat and Jyoti Mehta School of BiosciencesIndian Institute of Technology MadrasChennaiIndia
  2. 2.Department of Chemistry and Biotechnology, Faculty of Science, Engineering and TechnologySwinburne University of TechnologyHawthornAustralia

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