Structural characterization and applications of a novel polysaccharide produced by Azospirillum lipoferum MTCC 2306
- 159 Downloads
Azospirillum lipoferum MTCC 2306, a free-living nitrogen fixing bacteria, has a doubling time of 1.7 h in MPSS media. At the end of 28 h at a pH of 7 and temperature of 30 °C it produces 1.8 ± 0.013 g/L biomass and 2.1 ± 0.018 g/L of cyclic beta glucan (CβG) in MPSS medium with a yield coefficient (YP/S) of 2.1. This novel polysaccharide is a water-soluble cyclic biopolymer and is generally produced by Rhizobiaceae and predominantly made up of glucose. The CβG has a degree of polymerisation varying between 10 and 13 and has both α- and β-glycosidic linkages. It is not substituted with any functional groups such as acetates or succinates. Its ability to bind to aniline blue suggests that it can be a potential candidate for being used as carrier in medical imaging as well as in reducing toxicity of textile effluents. It is able to encapsulate rifampicin, a hydrophobic drug and increase its aqueous solubility by 71%. So, CβG appears to have promising applications in the field of drug, food, cosmetic and nutraceutical industries.
KeywordsAzospirillum lipoferum Rifampicin Cyclic β glucan Dye binding Encapsulation
The first author acknowledges the Ministry of Human Resource Development, Government of India for financial support. Department of Chemistry and Sophisticated analytical instrument facility (SAIF), IIT-Madras, for providing their analytical instrument facilities for characterization.
- Thaler S, Hofmann J, Bartz-Schmidt K-U, Schuettauf F, Haritoglou C, Yoeruek E (2011) Methyl blue and aniline blue versus patent blue and trypan blue as vital dyes in cataract surgery: capsule staining properties and cytotoxicity to human cultured corneal endothelial cells. J Cataract Refract Surg 37:1147–1153CrossRefGoogle Scholar
- Venkatachalam G, Narayanan S, Doble M (2012) Cyclic β-glucans from microorganisms: production, properties and applications. Springer, New YorkGoogle Scholar