Bioprospecting of whole-cell biocatalysts for cholesterol biotransformation
Microorganisms were isolated from industrial wool scouring effluents and from the soil adjacent to the wastewater treatment lagoon, both sterols-rich environments, in order to search for novel biocatalysts able to transform cholesterol. The isolates were identified on the basis of morphological and biochemical characteristics and phylogenetic analysis. Furthermore, a rapid and accurate bacteria identification by matrix assisted laser desorption/ionization-time-of-flight mass spectrometry was carried out. Bacteria and fungi including representatives of the genera Fusarium, Talaromyces, Trichoderma, Mucor, Aspergillus, Citrobacter, Proteus, Klebsiella, Exiguobacterium, Acinetobacter, Tsukamurella, Bacillus, and Streptomyces were found and evaluated for their ability to biotransform cholesterol by whole-cell treatment system. The results show that a Trichoderma koningiopsis strain, as well as two strains of Mucor circinelloides were able to transform cholesterol into value-added products. The major products were characterized as 7β-hydroxycholesterol, 4-cholesten-3-one, 5α,6α-epoxycholestan-3β-ol and 5β,6β-epoxycholestan-3β-ol. To the best of our knowledge, the present study is the first report of cholesterol biotransformation by representatives of Trichoderma and Mucor genera.
KeywordsBiotransformation Cholesterol MALDI-TOF MS Mucor Trichoderma Whole-cell biocatalyst
The authors are grateful to Lanasur SA for allowing them to take samples from the wool scouring wastewater treatment system. The authors thank Professor Anita Marsaioli and Dr. María Lair Sabóia, of Institute of Chemistry, University of Campinas; and Dr. Paula Rodríguez, Dr. Sonia Rodríguez, and Dr. Guillermo Moyna of Facultad de Química, Universidad de la República, for their assistance with this Project. This study was funded by Agencia Nacional de Investigación e Innovación (Award POS_NAC_2013_1_11432), Comisión Académica de Posgrado (CAP), Comisión Sectorial de Investigación Científica (CSIC), Programa de Desarrollo de las Ciencias Básicas (PEDECIBA), and Sociedad Uruguaya de Biociencias (SUB).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
- Bloch K (1991) Cholesterol: evolution of structure and function. In: Vance DE, Vance JE (eds) Biochemistry of lipids, lipoproteins and membranes. Elsevier B.V., Amsterdam, pp 363–381Google Scholar
- Pendharkar G, Anjum S, Patil S (2014) Enhanced biotransformation of phytosterols, a byproduct of soybean refineries, to key intermediate used for synthesis of steroidal drugs. Asian J Pharm Clin Res 7:178–180Google Scholar
- Soto-Castro D, Lara Contreras RC, Pina-Canseco M et al (2017) Solvent-free synthesis of 6β-phenylamino-cholestan-3β,5α-diol and (25R)-6β-phenylaminospirostan-3β,5α-diol as potential antiproliferative agents. Steroids 126:92–100. https://doi.org/10.1016/j.steroids.2017.08.008 CrossRefPubMedGoogle Scholar
- Wu K, Li W, Song J, Li T (2015) Production, purification, and identification of cholest- 4-en-3-one produced by cholesterol oxidase from Rhodococcus sp. in aqueous/organic biphasic system supplementary issue: ligand–receptor interactions and drug design. Biochem Insights 88:1–8. https://doi.org/10.4137/BCI.S21580 CrossRefGoogle Scholar