Relative expression of stress-related genes during acclimation at low temperature of psychrotrophic Oenococcus oeni strains from Patagonian wine

  • Nair Temis OlguínEmail author
  • Danay Valdés La Hens
  • Lucrecia Delfederico
  • Liliana Semorile
Original Paper


In the present study, we evaluated the transcriptional response of four stress-related genes in three Oenococcus oeni strains after acclimation at two different temperatures. Gene expression was analyzed at time zero and after 48 h acclimation at 18 and 21 °C. After the acclimation period cells were inoculated into sterile Pinot noir wine and MLF was followed for 25 days to investigate if different acclimation temperatures could influence cell survival and MLF performance. l-malic acid consumption, population survival, and transcriptional behavior were different upon the acclimation temperature. rmlB and hsp20 genes presented a considerable increase in their expression level when strains were acclimated at 18 °C particularly in the psychrotrophic strains UNQOe19 and UNQOe4 isolated from Patagonian Pinot noir wine in comparison with the control strain (ATCC 27310). The increase in rmlB and hsp20 expression could account for the better survival of these strains in Pinot noir in comparison with the control strain. In addition, Patagonian populations acclimated at 18 °C were able to consume a higher percentage of l-malic acid in comparison with cells acclimated at 21 °C. Our results suggest that gene expression analysis of cells acclimated at sub-optimal temperatures could benefit the selection of psychrotrophic strains aimed as starter cultures.


Acclimation Gene expression Malolactic fermentation Oenococcus oeni Psychrotrophic RT-qPCR 



This work was funded by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicada—Resolución (R) Nº 954/17); Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA, Proyecto PIT-AP-BA Nº 173/16); Consejo Interuniversitario Nacional—Consejo Nacional de Investigaciones Científicas y Técnicas (CIN—CONICET, PDTS 2014 Nº 173); and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, PICT 2014 Nº 1395, PICT 2017 Nº 1156). DVH and LS are members of the Research Career of CIC-BA; NTO is a member of the Research Career of CONICET.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y TecnologíaUniversidad Nacional de QuilmesBernalArgentina
  2. 2.Comisión de Investigaciones Científicas de la Provincia de Buenos Aires - CIC-BALa PlataArgentina
  3. 3.Consejo Nacional de Investigaciones Científicas y Técnicas – CONICETCiudad de Buenos AiresArgentina

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