Isolation and characterization of mineral-dissolving bacteria from different levels of altered mica schist surfaces and the adjacent soil
- 141 Downloads
Microorganisms play important role in mineral weathering. However, little is known about rock-associated mineral-dissolving bacteria. In this study, 129 bacterial isolates were obtained from the less and more weathered mica schist surfaces and the adjacent soil and characterized for mineral dissolving activity, population, and the linkage of rock weathering level and distribution of the bacteria. Among the 129 isolates, 112 isolates could dissolve biotite. The relative abundance of the highly effective Fe solubilizers was significantly higher on the more altered rock surface (89.6%) than in the soil (51.2%) and on the less altered rock surface (22.5%), while the relative abundance of the highly effective Si solubilizers was significantly higher in the soil (65.9%) than on the more (41.7%) and less (12.5%) altered rock surfaces. Furthermore, 17.5–42.5%, 87.5%, and 60.9–90.2% of the highly effective acid- and siderophore-producing isolates were obtained in the less and more weathered rocks and the soil, respectively. The mineral-dissolving bacteria belonged to 18 genera and Burkholderia, Bacillus, and Paenibacillus were the dominant and highly effective mineral-dissolving bacteria. Phylogenetic analysis found 2, 9, and 5 bacterial species in the highly effective mineral-dissolving bacteria on the less and more altered rock surfaces and in the soil, respectively. The results showed the abundant and diverse mineral-dissolving bacterial populations on the more weathered rock surfaces. The results also suggested distinct mineral-dissolving activities and mechanisms of the bacteria and highlighted the possibility for the development of bacterial inocula for plant nutrition improvement in silicate mineral-rich soils.
KeywordsMica schist Soil Mineral-dissolving bacteria Mineral dissolving activity and mechanism Population and diversity
This work was supported by the National Natural Science Foundation of China (41473075).
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–175Google Scholar
- Robert M, Berthelin J (1986) Role of biological and chemical factors in soil mineral weathering. In: Huang PM, Schnitzer M (eds) Interactions of soil minerals with natural organics and microbes. Special publication No 17, Soil Science Society of American, Madison, pp 453–495Google Scholar
- Sambrook J, Russell D (2001) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- Will C, Thurmer A, Wollherr A, Nacke H, Herold N, Schrumpf M, Gutknecht J, Wubet T, Buscot F, Daniel R (2010) Horizon-specific bacterial community community composition of german grassland soils, as revealed by pyrosequencing-based analysis of 16S rRNA gene. Appl Environ Microbiol 76:6751–6759CrossRefGoogle Scholar