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Diversity and seasonal distribution of ammonia-oxidizing archaea in the water column of a tropical estuary along the southeast Arabian Sea

  • Puthiya Veettil Vipindas
  • Thajudeen Jabir
  • Chekidhenkuzhiyil Jasmin
  • Tharakan Balu
  • Thekkendavida Velloth Rehitha
  • Balakrishnan Meenakshikutty Adarsh
  • Shanta Nair
  • Mohamed Hatha Abdulla
  • Anas Abdulaziz
Original Paper
  • 52 Downloads

Abstract

Diversity and distribution pattern of ammonia-oxidizing archaea (AOA) were studied across a salinity gradient in the water column of Cochin Estuary (CE), a tropical monsoonal estuary along the southeast Arabian Sea. The water column of CE was found to be nutrient rich with high bacterial (3.7–6.7 × 108 cells L−1) and archaeal abundance (1.9–4.5 × 108 cells L−1). Diversity and seasonal variation in the distribution pattern of AOA were studied using clone library analysis and Denaturing gradient gel electrophoresis (DGGE). Clone library analysis of both the amoA and 16S rRNA gene sequences showed similar diversity pattern, however the diversity was more clear when the 16S rRNA gene sequences were analyzed. More than 70% of the sequences retrieved were clustered under uncultured Thaumarchaeota group 1 lineage and the major fractions of the remaining sequences were grouped into the Nitrosopumilus lineage and Nitrosopelagicus lineage. The AOA community in the CE was less adaptable to changing environmental conditions and its distribution showed seasonal variations within the DGGE banding pattern with higher diversity during the pre-monsoon period. The distribution of AOA also showed its preference to intermediate salinity for their higher diversity. Summer monsoon associated runoff and flushing played a critical role in regulating the seasonality of AOA distribution.

Keywords

Ammonia oxidation Archaea Clone library DGGE Tropical estuary 

Notes

Acknowledgements

We are thankful to the Director, CSIR-National Institute of Oceanography (NIO), Goa, the Scientist-in-charge, CSIR-NIO, RC-Kochi and the Head, Department of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology for providing necessary facilities to carry out the work. The authors are grateful to COMAPS-ICMAM project and Centre for Marine Living Resources and Ecology, Ministry of Earth Sciences, Govt. of India for financial and technical support. The authors express their gratitude to Dr. C.T. Achuthankutty, Chief Scientist (Retired), CSIR-National Institute of Oceanography, Goa for critically reading the manuscript and improving its presentation.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Supplementary material

11274_2018_2570_MOESM1_ESM.doc (52 kb)
Supplementary material 1 (DOC 51 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Puthiya Veettil Vipindas
    • 1
    • 2
  • Thajudeen Jabir
    • 1
  • Chekidhenkuzhiyil Jasmin
    • 3
  • Tharakan Balu
    • 3
  • Thekkendavida Velloth Rehitha
    • 3
  • Balakrishnan Meenakshikutty Adarsh
    • 1
  • Shanta Nair
    • 3
  • Mohamed Hatha Abdulla
    • 1
  • Anas Abdulaziz
    • 3
  1. 1.Department of Marine Biology, Microbiology and Biochemistry, School of Marine SciencesCochin University of Science and TechnologyCochinIndia
  2. 2.Cryobiology LaboratoryNational Centre for Polar and Ocean ResearchVasco-da-GamaIndia
  3. 3.Council of Scientific and Industrial Research (CSIR) - National Institute of Oceanography, Regional CentreCochinIndia

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