Isolation and characterization of aerobic, culturable, arsenic-tolerant bacteria from lead–zinc mine tailing in southern China
Bioremediation of arsenic (As) pollution is an important environmental issue. The present investigation was carried out to isolate As-resistant novel bacteria and characterize their As transformation and tolerance ability. A total of 170 As-resistant bacteria were isolated from As-contaminated soils at the Kangjiawan lead–zinc tailing mine, located in Hunan Province, southern China. Thirteen As-resistant isolates were screened by exposure to 260 mM Na2HAsO4·7H2O, most of which showed a very high level of resistance to As5+ (MIC ≥ 600 mM) and As3+ (MIC ≥ 10 mM). Sequence analysis of 16S rRNA genes indicated that the 13 isolates tested belong to the phyla Firmicutes, Proteobacteria and Actinobacteria, and these isolates were assigned to eight genera, Bacillus, Williamsia, Citricoccus, Rhodococcus, Arthrobacter, Ochrobactrum, Pseudomonas and Sphingomonas. Genes involved in As resistance were present in 11 of the isolates. All 13 strains transformed As (1 mM); the oxidation and reduction rates were 5–30% and 10–51.2% within 72 h, respectively. The rates of oxidation by Bacillus sp. Tw1 and Pseudomonas spp. Tw224 peaked at 42.48 and 34.94% at 120 h, respectively. For Pseudomonas spp. Tw224 and Bacillus sp. Tw133, the highest reduction rates were 52.01% at 48 h and 48.66% at 144 h, respectively. Our findings will facilitate further research into As metabolism and bioremediation of As pollution by genome sequencing and genes modification.
KeywordsAs-resistant bacteria As pollution Oxidation Reduction 16S rRNA analysis
This work was supported by the Fundamental Research Funds for the Central Universities (Grant Nos. 2016JX03, YX2014-15), the National Science and Technology Ministry (Grant No. 2012BAC09B03), the National Natural Science Foundation of China (Grant No. J1310005) and the Beijing Nova Program (Grant No. 2011033).
Compliance with ethical standards
Conflict of interest
All authors declare no conflicts of interest.
- Abedin MJ, Cotter-Howells J, Meharg AA (2002) Arsenic uptake and accumulation in rice (Oryza sativa L.) irrigated with contaminated water. Plant Soil 240(2):311–319Google Scholar
- Cai L, Liu GH, Rensing C, Wang GJ (2009) Genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. BMC Microbiol 9(4):1–11Google Scholar
- Cordi A, Pagnout C, Devin S, Poirel J, Billard P, Dollard MA, Bauda P (2015) Determination of physiological, taxonomic, and molecular characteristics of a cultivable arsenic-resistant bacterial community. Environ Sci Poll Res 22(18):13753–13763Google Scholar
- Jackson CR, Dugas SL (2003) Phylogenetic analysis of bacterial and archaeal arsC gene sequences suggests an ancient, common origin for arsenate reductase. BMC Evol Biol 3(1):1–10Google Scholar
- Katsoyianni I, Zouboulis A, Althoff H, Bartel H (2002) As(III)removal from groundwaters using fixed-bed upflow bioreactors. Chemosphere 47(3):325–332Google Scholar
- Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Sys Evol Microbiol 62(3):716–721Google Scholar
- Krumova K, Nikolovska M, Groudeva V (2008) Isolation and identification of arsenic-transforming bacteria from arsenic-contaminated sites in Bulgaria. Biotechnol Biotechnol Equip 22(2):721–728Google Scholar
- Lett MC, Paknikar KM, Lievremont D (2001) A simple and rapid method for arsenite and arsenate speciation. Process Met 11:541–546Google Scholar
- Luo X, Liu H, Huang G, Li Y, Zhao Y, Li X (2015) Remediation of arsenic-contaminated groundwater using media-injected permeable reactive barriers with a modified montmorillonite: sand tank studies. Environ Sci Poll Res 23(1):870–877Google Scholar
- Paul D, Poddar S, Sar P (2014) Characterization of arsenite-oxidizing bacteria isolated from arsenic-contaminated groundwater of West Bengal. J Environ Sci Health Part A 49(13):1481–1492Google Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425Google Scholar
- Sanyal SK, Mou TJ, Chakrabarty RP, Hoque S, Hossain MA, Sultanal M (2016) Diversity of arsenite oxidase gene and arsenotrophic bacteria in arsenic-affected Bangladesh soils. AMB Express 6(1):1–11Google Scholar
- Shagol CC, Krishnamoorthy R, Kim K, Sundaram S, Sa T (2014) Arsenic-tolerant plant-growth-promoting bacteria isolated from arsenic-polluted soils in South Korea. Environ Sci Poll Res 21(15):9356–9365Google Scholar
- Sun YM, Polishchuk EA, Radoja U, Cullen WR (2004) Identification and quantification of arsC genes in environmental samples by using real-time PCR. J Microbial Methods 58(3):335–349Google Scholar
- Teclu D, Tivchev G, Laing M, Wallis M (2009) Bioremoval of arsenic species from contaminated waters by sulphate–reducing bacteria. Water Res 42:4885–4893Google Scholar
- Wu F, Wang J, Yang J, Li J, Zheng Y (2016) Does arsenic play an important role in the soil microbial community around a typical arsenic mining area. Environ Poll 213:949–956Google Scholar