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Evidence of genetically diverse virulent mating types of Phytophthora capsici from Capsicum annum L.

  • Kiran Nawaz
  • Ahmad Ali Shahid
  • Louis Bengyella
  • Muhammad Nasir Subhani
  • Muhammad Ali
  • Waheed Anwar
  • Sehrish Iftikhar
  • Shinawar Waseem Ali
Original Paper

Abstract

Chili pepper (Capsicum annum L.) is an important economic crop that is severely destroyed by the filamentous oomycete Phytophthora capsici. Little is known about this pathogen in key chili pepper farms in Punjab province, Pakistan. We investigated the genetic diversity of P. capsici strains using standard taxonomic and molecular tools, and characterized their colony growth patterns as well as their disease severity on chili pepper plants under the greenhouse conditions. Phylogenetic analysis based on ribosomal DNA (rDNA), β-tubulin and translation elongation factor 1α loci revealed divergent evolution in the population structure of P. capsici isolates. The mean oospore diameter of mating type A1 isolates was greater than that of mating type A2 isolates. We provide first evidence of an uneven distribution of highly virulent mating type A1 and A2 of P. capsici that are insensitive to mefenoxam, pyrimorph, dimethomorph, and azoxystrobin fungicides, and represent a risk factor that could ease outpacing the current P. capsici management strategies.

Keywords

Phylogeny β-Tubulin Pathogenicity test Oomycetes Root-rot Fungicide resistance 

Notes

Acknowledgements

This project was sponsored by the Higher Education Commission of Pakistan; via competitive Indigenous PhD fellowship program No: 112-34924-2AV1-197. We thank all the farm owners that allowed regular visits to their farms.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

11274_2018_2511_MOESM1_ESM.docx (22 kb)
Supplementary material 1 (DOCX 21 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Kiran Nawaz
    • 1
  • Ahmad Ali Shahid
    • 1
    • 2
  • Louis Bengyella
    • 3
    • 4
  • Muhammad Nasir Subhani
    • 1
  • Muhammad Ali
    • 1
  • Waheed Anwar
    • 1
  • Sehrish Iftikhar
    • 1
  • Shinawar Waseem Ali
    • 1
  1. 1.Institute of Agricultural ScienceUniversity of the PunjabLahorePakistan
  2. 2.Centre of Excellence in Molecular BiologyUniversity of the PunjabLahorePakistan
  3. 3.Tree Fruit Research and Extension Center (TFREC), College of Agricultural, Human and Natural Resource Sciences (CAHNRS)Washington State UniversityWenatcheeUSA
  4. 4.Department of Biological ControlAdvanced Biotech CooperativeBali-NyongaCameroon

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