Environmental proteomic studies: closer step to understand bacterial biofilms
Advancement in proteome analytical techniques and the development of protein databases have been helping to understand the physiology and subtle molecular mechanisms behind biofilm formation in bacteria. This review is to highlight how the evolving proteomic approaches have revealed fundamental molecular processes underlying the formation and regulation of bacterial biofilms. Based on the survey of research reports available on differential expression of proteins in biofilms of bacterial from wide range of environments, four important cellular processes viz. metabolism, motility, transport and stress response that contribute to formation of bacterial biofilms are discussed. This review might answer how proteins related to these cellular processes contribute significantly in stabilizing biofilms of different bacteria in diverse environmental conditions.
KeywordsBacteria Biofilm Proteomics Tools Techniques
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
Two dimensional polyacrylamide gel electrophoresis
Isotope coded affinity tag
Isobaric tag for relative and absolute quantitation
Tandem mass tags
Acid mine drainage
- 3D, OD
Difference gel electrophoresis
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry
Electrospray ionization-quadrupole ion trap mass spectrometry
Nano liquid chromatography-mass spectrometry
- LC-ESI, TCA
Tricarboxylic acid cycle
Bis-(3′-5′)-cyclic dimeric guanosine monophosphate
Tripartite ATP-independent periplasmic
Reactive oxygen species
Outer membrane vesicles
The authors gratefully acknowledge the support rendered by the management of Vellore Institute of Technology, Vellore, India in carrying out their research.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interests.
Research involving human participants and/or animals
The research work does not involve human participants and/or animals.
The research work does not involve human participants and hence informed consent does not arise.
- Babin BM, Bergkessel M, Sweredoskie MJ, Moradiane A, Hesse S, Newman DK, Tirrel DA (2015) SutA is a bacterial transcription factor expressed during slow growth in Pseudomonas aeruginosa. Proc Natl Acad Sci USA 103:2833–2838Google Scholar
- Chua SL, Yam JKH, Hao P, Adav SS, Salido MM, Liu Y, Givskov M, Sze SK, Nielsen TT, Yang L (2016) Selective labeling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms. Nat Commun 7:10750. https://doi.org/10.1038/ncomms10750 CrossRefPubMedPubMedCentralGoogle Scholar
- Freiberg JA, Le Breton Y, Tran BQ, Scott AJ, Harro JM, Ernst RK, Goo YA, Mongodin EF, Goodlett DR, McIver KS, Shirtliff ME (2016) Global analysis and comparison of the transcriptomes and proteomes of group A Streptococcus biofilms. mSystems 1(6):e00149–e00116CrossRefPubMedPubMedCentralGoogle Scholar
- Lassek C, Burghartz M, Chaves-Moreno D, Otto A, Hentschker C, Fuchs S, Bernhardt J, Jauregui R, Neubauer R, Becher D, Pieper DH, Jahn M, Jahn D, Riedel K (2015) A metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). Mol Cell Proteom 14(4):989–1008CrossRefGoogle Scholar
- Oosthuizen MC, Steyn B, Lindsay DJ, Brözel VS, Cosette P, von Holy A (2001) Novel method for the proteomic investigation of a dairy-associated Bacillus cereus biofilm. Appl Environ Microbiol 194:47–51Google Scholar
- Sethupathy S, Prasath KG, Ananthi S, Mahalingam S, Balan SY, Pandian SK (2016) Proteomic analysis reveals modulation of iron homeostasis and oxidative stress response in Pseudomonas aeruginosa PAO1 by curcumin inhibiting quorum sensing regulated virulence factors and biofilm production. J Proteom 145:112–126CrossRefGoogle Scholar
- Silva AF, Dos Santos AR, Coelho Trevisan DA, Ribeiro AB, Zanetti Campanerut-Sá PA, Kukolj C, de Souza EM, Cardoso RF, Estivalet Svidzinski TI, de Abreu Filho BA, Junior MM, Graton Mikcha JM (2018) Cinnamaldehyde induces changes in the protein profile of Salmonella Typhimurium biofilm. Res Microbiol 169:33–43CrossRefPubMedGoogle Scholar
- Vaysse PJ, Prat L, Mangenot S, Cruveiller S, Goulas P, Grimaud R (2009) Proteomic analysis of Marinobacter hydrocarbonoclasticus SP17 biofilm formation at the alkane-water interface reveals novel proteins and cellular processes involved in hexadecane assimilation. Res Microbiol 160:829–837CrossRefPubMedGoogle Scholar
- Wick LM, Quadroni M, Egli T (2001) Short- and long-term changes in proteome composition and kinetic properties in a culture of Escherichia coli during transition from glucose-excess to glucose-limited growth conditions in continuous culture and vice versa. Environ Microbiol 3:588–599CrossRefPubMedGoogle Scholar