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Coexistence of quorum-quenching and quorum-sensing in tropical marine Pseudomonas aeruginosa strain MW3A

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Abstract

A chemically defined medium called KGm medium was used to isolate from a sample of sea water a bacterial strain, MW3A, capable of using N-3-oxohexanoyl-l-homoserine lactone as the sole carbon source. MW3A was clustered closely to Pseudomonas aeruginosa by 16S ribosomal DNA sequence analysis. It degraded both N-acylhomoserine lactones (AHLs) with a 3-oxo group substitution and, less preferably, AHLs with unsubstituted groups at C3 position in the acyl side chain, as determined by Rapid Resolution Liquid Chromatography. Its quiP and pvdQ homologue gene sequences showed high similarities to those of known acylases. Spent supernatant of MW3A harvested at 8-h post inoculation was shown to contain long-chain AHLs when assayed with the biosensor Escherichia coli [pSB1075], and specifically N-dodecanoyl-l-homoserine lactone and N-3-oxotetradecanoyl-l-homoserine lactone by high resolution mass spectrometry. Hence, we report here a novel marine P. aeruginosa strain MW3A possessing both quorum-quenching and quorum-sensing properties.

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Acknowledgments

This work was supported by a High Impact Research Grant (J-00000-73552) and the Malaysia Ministry of Science, Technology and Innovation under the Science Fund (12-02-03-2085) to KG Chan and a Postgraduate Research Grant (PS256/2008A) to CS Wong from the University of Malaya.

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Authors and Affiliations

  1. Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Cheng-Siang Wong, Wai-Fong Yin & Kok-Gan Chan

  2. Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Yeun-Mun Choo

  3. Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore, 637616, Singapore

    Choon-Kook Sam & Chong-Lek Koh

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  1. Cheng-Siang Wong
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  2. Wai-Fong Yin
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  3. Yeun-Mun Choo
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  4. Choon-Kook Sam
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  5. Chong-Lek Koh
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  6. Kok-Gan Chan
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Correspondence to Kok-Gan Chan.

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Wong, CS., Yin, WF., Choo, YM. et al. Coexistence of quorum-quenching and quorum-sensing in tropical marine Pseudomonas aeruginosa strain MW3A. World J Microbiol Biotechnol 28, 453–461 (2012). https://doi.org/10.1007/s11274-011-0836-x

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