Virus Genes

, Volume 55, Issue 4, pp 557–561 | Cite as

Characterization of a new bunyavirus and its derived small RNAs in the brown citrus aphid, Aphis citricidus

  • Wei Zhang
  • Tengfei Wu
  • Mengmeng Guo
  • Tengyu Chang
  • Li Yang
  • Yang Tan
  • Chao Ye
  • Jinzhi Niu
  • Jin-Jun WangEmail author
Short Report


High-throughput sequencing is widely used for virus discovery, and many RNA viruses have been discovered and identified. A new negative-sense single-stranded RNA virus was identified in the brown citrus aphid and named Aphis citricidus bunyavirus. The genome consists of large (7037 nt), medium (3462 nt), and small (1163 nt) segments. Phylogenetic analysis and amino acid sequences identities of this virus with other bunyaviruses suggest that it is a new species belonging to the family Phenuiviridae. The small interfering RNA pathway could be involved against the infection of this virus in brown citrus aphid as supported by the viral derived small RNAs. The discovery of this virus illustrates the diversity of RNA viruses and contributes to the classification of bunyaviruses.


Bunyaviruses Aphids RNAi Small RNA 



The authors acknowledge the support of the National Natural Science Foundation of China (31701846) and the earmarked fund for Modern Agro-industry (Citrus) Technology Research System of China (CARS-26). J.N. is a recipient of China Postdoctoral Science Foundation Grant (2018T110939) and Postdoctoral Special Research Funds of Chongqing Municipal (Xm2017017).

Author contributions

WZ, JN and JW designed the study. The experiments are performed by WZ, TW, TC, LY, YT and CY. WZ, TW and TC contributed to sequencing data and PCR analysis. TC, LY, YT, MG performed the alignment and phylogenetic tree, MG contributed to test Aphis citricidus bunyavirus in citrus. WZ wrote the initial draft. JN and JW edited the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

11262_2019_1667_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1087 kb)


  1. 1.
    Contigiani MS, Diaz LA, Tauro LB (2017) Bunyaviruses. In: Marcondes C (ed) Arthropod borne diseases. Springer, Cham, pp 137–154Google Scholar
  2. 2.
    Walter CT, Barr JN (2011) Recent advances in the molecular and cellular biology of bunyaviruses. J Gen Virol 92:2467–2484CrossRefPubMedGoogle Scholar
  3. 3.
    Webster CG, Reitz SR, Perry KL, Adkins S (2011) A natural M RNA reassortant arising from two species of plant- and insect-infecting bunyaviruses and comparison of its sequence and biological properties to parental species. Virology 413:216–225CrossRefPubMedGoogle Scholar
  4. 4.
    Blackman RL, Sorin M, Miyazaki M (2011) Sexual morphs and colour variants of Aphis (formerly Toxoptera) odinae (Hemiptera, Aphididae) in Japan. Zootaxa 3110:53–60CrossRefGoogle Scholar
  5. 5.
    Hunter WB, Dang PM, Bausher MG, Chaparro JX, McKendree W, Shatters RG Jr, McKenzie CL, Sinisterra XH (2003) Aphid biology: expressed genes from alate Toxoptera citricida, the brown citrus aphid. J Insect Sci 3:23CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Moreno P, Ambros S, Albiach-Marti MR, Guerri J, Pena L (2008) Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Mol Plant Pathol 9:251–268CrossRefPubMedGoogle Scholar
  7. 7.
    Liu S, Vijayendran D, Bonning BC (2011) Next generation sequencing technologies for insect virus discovery. Viruses 3:1849–1869CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Van den Heuvel JF, Hummelen H, Verbeek M, Wilk AM, Van der Heuvel F (1997) Characteristics of Acyrthosiphon pisum Virus, a newly identified virus infecting the pea aphid. J Invertebr Pathol 70:169–176CrossRefPubMedGoogle Scholar
  9. 9.
    Liu S, Vijayendran D, Chen Y, Bonning BC (2016) Aphis Glycines Virus 2, a novel insect virus with a unique genome structure. Viruses 8:315CrossRefPubMedCentralGoogle Scholar
  10. 10.
    Alcala-Briseno RI, Okada R, Herrera F, Valverde RA (2018) A novel endornavirus isolated from cluster bean (Cyamopsis tetragonoloba). Arch Virol 163:2279–2282CrossRefPubMedGoogle Scholar
  11. 11.
    Ryabov EV, Keane G, Naish N, Evered C, Winstanley D (2009) Densovirus induces winged morphs in asexual clones of the rosy apple aphid, Dysaphis plantaginea. Proc Natl Acad Sci USA 106:8465–8470CrossRefPubMedGoogle Scholar
  12. 12.
    Ryabov EV (2007) A novel virus isolated from the aphid Brevicoryne brassicae with similarity to Hymenoptera picorna-like viruses. J Gen Virol 88:2590–2595CrossRefPubMedGoogle Scholar
  13. 13.
    Shang F, Wei DD, Jiang XZ, Wei D, Shen GM, Feng YC, Li T, Wang JJ (2015) Reference gene validation for quantitative PCR under various biotic and abiotic stress conditions in Toxoptera citricida (Hemiptera, Aphidiae). J Econ Entomol 108:2040–2047CrossRefPubMedGoogle Scholar
  14. 14.
    Reguera J, Weber F, Cusack S (2010) Bunyaviridae RNA polymerases (L-protein) have an N-terminal, influenza-like endonuclease domain, essential for viral cap-dependent transcription. PLoS Pathog 6:e1001101CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Amroun A, Priet S, de Lamballerie X, Querat G (2017) Bunyaviridae RdRps: structure, motifs, and RNA synthesis machinery. Crit Rev Microbiol 43:753–778CrossRefPubMedGoogle Scholar
  16. 16.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Niu J, Smagghe G, De Coninck DI, Van Nieuwerburgh F, Deforce D, Meeus I (2016) In vivo study of Dicer-2-mediated immune response of the small interfering RNA pathway upon systemic infections of virulent and avirulent viruses in Bombus terrestris. Insect Biochem Mol Biol 70:127–137CrossRefPubMedGoogle Scholar
  18. 18.
    Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW (2004) Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 117:69–81CrossRefPubMedGoogle Scholar
  19. 19.
    Niu JZ, Taning CNT, Christiaens O, Smagghe G, Wang JJ (2018) Rethink RNAi in insect pest control: challenges and perspectives. Adv Insect Physiol 55:1–17CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Entomology and Pest Control Engineering, College of Plant ProtectionSouthwest UniversityChongqingChina
  2. 2.International Joint Laboratory on China-Belgium Sustainable Crop Pest Control, Academy of Agricultural SciencesSouthwest UniversityChongqingChina

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