Characterization of a new bunyavirus and its derived small RNAs in the brown citrus aphid, Aphis citricidus
High-throughput sequencing is widely used for virus discovery, and many RNA viruses have been discovered and identified. A new negative-sense single-stranded RNA virus was identified in the brown citrus aphid and named Aphis citricidus bunyavirus. The genome consists of large (7037 nt), medium (3462 nt), and small (1163 nt) segments. Phylogenetic analysis and amino acid sequences identities of this virus with other bunyaviruses suggest that it is a new species belonging to the family Phenuiviridae. The small interfering RNA pathway could be involved against the infection of this virus in brown citrus aphid as supported by the viral derived small RNAs. The discovery of this virus illustrates the diversity of RNA viruses and contributes to the classification of bunyaviruses.
KeywordsBunyaviruses Aphids RNAi Small RNA
The authors acknowledge the support of the National Natural Science Foundation of China (31701846) and the earmarked fund for Modern Agro-industry (Citrus) Technology Research System of China (CARS-26). J.N. is a recipient of China Postdoctoral Science Foundation Grant (2018T110939) and Postdoctoral Special Research Funds of Chongqing Municipal (Xm2017017).
WZ, JN and JW designed the study. The experiments are performed by WZ, TW, TC, LY, YT and CY. WZ, TW and TC contributed to sequencing data and PCR analysis. TC, LY, YT, MG performed the alignment and phylogenetic tree, MG contributed to test Aphis citricidus bunyavirus in citrus. WZ wrote the initial draft. JN and JW edited the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Contigiani MS, Diaz LA, Tauro LB (2017) Bunyaviruses. In: Marcondes C (ed) Arthropod borne diseases. Springer, Cham, pp 137–154Google Scholar
- 17.Niu J, Smagghe G, De Coninck DI, Van Nieuwerburgh F, Deforce D, Meeus I (2016) In vivo study of Dicer-2-mediated immune response of the small interfering RNA pathway upon systemic infections of virulent and avirulent viruses in Bombus terrestris. Insect Biochem Mol Biol 70:127–137CrossRefPubMedGoogle Scholar