The BeWo cell line derived from a human placental choriocarcinoma is permissive for respiratory syncytial virus infection
The respiratory syncytial virus (RSV) is the main pathogen associated with upper respiratory tract infections during early childhood. Vertical transmission of this virus has been suggested in humans, based on observations recorded during animal studies that revealed an association of RSV with persistent structural and functional changes in the developing lungs of the offspring. However, human placentas have not yet been evaluated for susceptibility to RSV infection. In this study, we examined the capacity of RSV to infect a human trophoblast model, the BeWo cell line. Our results suggest that BeWo cells are susceptible to RSV infection since they allow RNA viral replication, viral protein translation, leading to the production of infectious RSV particles. In this report, we demonstrate that a human placenta model system, consisting of BeWo cells, is permissive to RSV infection. Thus, the BeWo cell line may represent a useful model for studies that aim to characterize the events of a possible RSV infection at the human maternal–fetal interface.
KeywordsRespiratory syncytial virus BeWo cells Trophoblast Placenta Viral cycle replication
We are grateful to Dr. Beatriz Gomez (UNAM), Dr. Alberto Guzman (INPer), Dra. Veronica Zaga Clavellina (INPer), Dr. Lorena Gutierrez (Cinvestav), and Dr. Yonathan Garfias (UNAM) for the antibodies, cells, and viruses donated for this study. This research was supported by the National Council on Science and Technology CONACYT (CB-2015-01-255007 to M.L.J.) and Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” (212250-3210-21007-03-15 to M.L.J.). L.D.G.G. received a fellowship from CONACYT. Additionally, M.L.J., M.H.I., G.C.J., and H.R.A.C. acknowledge their membership of the National System of Researchers (SNI).
Conceived and designed the experiments: MAVC, JGC, HRAC, and MLJ. Performed the experiments: MAVC, LDGG, TAVP, MSMG and MHI. Contributed reagents/materials/analysis tools: MHI and HRAC. Wrote the paper: MLJ, MMC, and GLR. Coordinated and facilitated the project: MLJ. All authors have read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that no conflicts of interest exist.
Research involving human participants or animals
This article does not contain any study with human participants or animals, performed by any of the authors.
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