Molecular characterization of a new recombinant brassica yellows virus infecting tobacco in China
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Brassica yellows virus (BrYV), prevalently distributed throughout mainland China and South Korea while triggering serious diseases in cruciferous crops, is proposed to be a new species in the genus Polerovirus within the family Luteoviridae. There are three distinct genotypes (BrYV-A, BrYV-B and BrYV-C) reported in cabbage and radish. Here, we describe a new BrYV isolate infecting tobacco plants in the field, which was named BrYV-NtabQJ. The complete genome sequence of BrYV-NtabQJ is 5741 nt in length, and 89% of the sequence shares higher sequence identities (about 90%) with different BrYV isolates. However, it possesses a quite divergent region within ORF5, which is more close to Beet western yellows virus (BWYV), Beet mild yellowing virus (BMYV) and Beet chlorosis virus (BChV). A significant recombination event was then detected among BrYV-NtabQJ, BrYV-B Beijng isolate (BrYV-BBJ) and BWYV Leonurus sibiricus isolate (BWYV-LS). It is proposed that BrYV-NtabQJ might be an interspecific recombinant between BrYV-BBJ and BWYV-LS, and the recombination might result in the successful aphid transmission of BrYV from cruciferous crops to tobacco. And it also poses new challenges for BrYV diagnosis and the vegetable production.
KeywordsBrassica yellows virus Recombination Tobacco Polerovirus Phylogenic analysis
This work was supported by the Science Foundation for Young Scholars of Tobacco Research Institute of Chinese Academy of Agricultural Sciences (2016B02), Natural Science Foundation of Shandong Province (ZR2017QC003) and the Fundamental Research Funds for China Agricultural Academy of Sciences (1610232016005).
This work was conceived, designed, performed and written by QW. FX and LA contributed to the sample collection and virus detection. HX and WZ provided valuable suggestions to the detection methods. GL and HL participated in its design and coordination. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Research involving human participants or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
- 2.Sztuba-Solińska J, Urbanowicz A, Figlerowicz M, Bujarski JJ (2011) RNA-RNA recombination in plant virus replication and evolution. Annu Rev Phytopathol 49:415–443. https://doi.org/10.1146/annurev-phyto-072910-095351 CrossRefPubMedGoogle Scholar
- 10.Zhang X, Peng Y, Wang Y, Zhang Z, Li D, Yu J, Han C (2016) Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription-polymerase chain reaction. Virol J 13:189–189. https://doi.org/10.1186/s12985-016-0647-7 CrossRefPubMedPubMedCentralGoogle Scholar
- 14.Brault V, Périgon S, Reinbold C, Erdinger M, Scheidecker D, Herrbach E, Richards K, Ziegler-Graff V (2005) The polerovirus minor capsid protein determines vector specificity and intestinal tropism in the aphid. J Virol 79:9685–9693. https://doi.org/10.1128/jvi.79.15.9685-9693.2005 CrossRefPubMedPubMedCentralGoogle Scholar
- 15.Knierim D, Deng TC, Tsai WS, Green SK, Kenyon L (2010) Molecular identification of three distinct Polerovirus species and a recombinant Cucurbit aphid-borne yellows virus strain infecting cucurbit crops in Taiwan. Plant Pathol 59:991–1002. https://doi.org/10.1111/j.1365-3059.2010.02327.x CrossRefGoogle Scholar
- 17.Moonan F, Mirkov TE (2002) Analyses of genotypic diversity among North, South, and Central American isolates of Sugarcane yellow leaf virus: evidence for Colombian origins and for intraspecific spatial phylogenetic variation. J Virol 76:1339–1348. https://doi.org/10.1128/jvi.76.3.1339-1348.2002 CrossRefPubMedPubMedCentralGoogle Scholar