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Virus Genes

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Triticum aestivum heat shock protein 23.6 interacts with the coat protein of wheat yellow mosaic virus

  • Shanshan Jiang
  • Bin Wu
  • Liangliang Jiang
  • Mei Zhang
  • Yuwen Lu
  • Shengji Wang
  • Fei YanEmail author
  • Xiangqi XinEmail author
Article
  • 67 Downloads

Abstract

The role of heat shock proteins (HSPs) in viral replication has been described in numerous publications. Wheat yellow mosaic virus (WYMV) belongs to the genus Bymovirus (family Potyviridae), which causes yellow mosaic and dwarf symptoms in wheat (Triticum aestivum). In this study, the T. aestivum heat shock protein 23.6 (TaHSP23.6), which belongs to the small heat shock protein family, was shown to interact with the WYMV coat protein (CP) in a yeast two-hybrid screen. The co-localization and interaction between TaHSP23.6 and WYMV CP were additionally verified in Nicotiana benthamiana by co-localization assays and bimolecular fluorescence complementation (BiFC). Not only the transcription of TaHSP23.6 but also that of other HSP family members (TaHSP70, TaHSP90, and TaHSP101) was up-regulated in WYMV-infected leaves, as shown by semi-quantitative PCR assays. Interestingly, the expression levels of the T. aestivum heat stress transcription factor A2 (TaHSFA2) members were varied in response to WYMV infection. Thus, our results provide insights into the interaction between TaHSP23.6 and WYMV infection.

Keywords

Wheat yellow mosaic virus Triticum aestivum heat shock protein 23.6 Coat protein Interaction Chaperone 

Notes

Acknowledgements

We would like to thank all the laboratory technicians for their contribution to this research. This work was supported by the National Natural Science Foundation of China (31701762), the Natural Science Foundation of Shandong (BS2015SW015), the Open Fund of State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control (2010DS700124-KF1703), and the National Key R&D Program of China (2017YFD0201700).

Author contributions

SJ, FY, and XX conceived, designed, and performed the experiments; SJ, LJ, and YL helped perform the experiments; BW and MZ analyzed the date; SW was involved in the survey and sample collection; SW and XX contributed reagents/materials/analysis tools; and SJ wrote the manuscript. All authors approved the final draft of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not include any studies with human participants or animals performed by any of the authors.

Informed consent

Consent to submit has been received explicitly from all co-authors, as well as from the responsible authorities—tacitly or explicitly—at the institute/organization where the work has been carried out before the work is submitted.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shanshan Jiang
    • 1
  • Bin Wu
    • 1
  • Liangliang Jiang
    • 2
  • Mei Zhang
    • 1
  • Yuwen Lu
    • 2
  • Shengji Wang
    • 1
  • Fei Yan
    • 2
    Email author
  • Xiangqi Xin
    • 1
    Email author
  1. 1.Shandong Province Key Laboratory of Plant Virology, Institute of Plant ProtectionShandong Academy of Agricultural SciencesJinanChina
  2. 2.Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture, Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhouChina

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