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Virus Genes

, Volume 55, Issue 2, pp 257–265 | Cite as

Isolation and characterization of bacteriophage NTR1 infectious for Nocardia transvalensis and other Nocardia species

  • Stephanie Taylor
  • Teagan L. Brown
  • Joseph Tucci
  • Peter Lock
  • Robert J. Seviour
  • Steve PetrovskiEmail author
Article

Abstract

We describe here the isolation and characterization of the bacteriophage, NTR1 from activated sludge. This phage is lytic for Nocardia transvalensis, Nocardia brasiliensis and Nocardia farcinica. NTR1 phage has a genome sequence of 65,275 bp in length, and its closest match is to the Skermania piniformis phage SPI1 sharing over 36% of its genome. The phage belongs to the Siphoviridae family, possessing a long non-contractile tail and icosahedral head. Annotation of the genome reveals 97 putative open reading frames arranged in the characteristic modular organization of Siphoviridae phages and contains a single tRNA-Met gene.

Keywords

Bacteriophage Nocardia phage Genome sequence Phage therapy Siphoviridae 

Notes

Acknowledgements

We would like to acknowledge the LIMS BioImaging Facility for use of the Electron Microscope and the La Trobe University Genomics Platform.

Author contributions

JT, RJS and SP conceived the study as part of a larger project. SP commenced the project and ST and TLB performed the sequencing and annotation. PL generated the transmission electron microscope image. ST and SP performed the DNA sequence analysis and wrote the manuscript. All authors approved the manuscript.

Funding

The Petrovski laboratory receives supported from the La Trobe University, Research Focus Area (RFA) securing food, water and the environment.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Physiology, Anatomy & MicrobiologyLa Trobe UniversityBundooraAustralia
  2. 2.Department of Pharmacy & Applied Science, La Trobe Institute for Molecular ScienceLa Trobe UniversityBendigoAustralia
  3. 3.La Trobe Institute for Molecular SciencesLa Trobe UniversityBundooraAustralia

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