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Virus Genes

pp 1–9 | Cite as

Development of a reverse genetics system for a feline panleukopenia virus

  • Nan Cheng
  • Yongkun Zhao
  • Qiuxue Han
  • Weijiao Zhang
  • Ji Xi
  • Yongle Yu
  • Hualei Wang
  • Guohua Li
  • Yuwei Gao
  • Songtao Yang
  • Weiquan Liu
  • Xianzhu Xia
Article

Abstract

Feline panleukopenia virus (FPV) infects cats and can be fatal to kittens. There is evidence that canine parvovirus originated from FPV, which makes FPV important in studies of the family Parvoviridae. In the present study, the entire genome of FPV strain HH-1/86 was converted into a full-length infectious clone (pFPV). The FPV strain HH-1/86 has a 5123-nt single stranded DNA genome with a Y-shaped inverted 3′ terminal repeat (ITR) and a U-shaped inverted 5′ ITR. Feline kidney cells (F81) were transfected with the pFPV clone which contained a genetic marker, and a rescued virus was obtained (rFPV). The rFPV was identified by its cytopathic effects, indirect immunofluorescence, growth curve analysis, western blot assay and hemagglutination, and was indistinguishable from the parent virus. The FPV infectious clone will facilitate the study of pathogenicity and viral replication of FPV and the inter-species transmission of parvoviruses.

Keywords

Feline panleukopenia virus Infectious clone Rescue virus Biological properties 

Notes

Acknowledgements

This work was supported in part by the National Key Research and Development Program of China (No. 2016YFD0501004).

Author contributions

SY, YZ and WL conceived and designed the experiments. NC and WZ performed the experiments. JX, YY, HW, YG, and XX analyzed the data. NC and WZ wrote the article. GL managed and submitted the paper.

Funding

Funding was provided by National Natural Science Foundation of Jilin Province and Public Welfare (Agricultural) Industry Research Special Program.

Compliance with ethical standards

Conflict of interest

The authors do not have any conflicts of interest.

Ethical approval

This study was carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals developed by the China Council on Animal Care. All protocols were approved by the Committee on the Ethics of Animal Experiments of the Institute of Military Veterinary at the Academy of Military Medical Sciences. The approved ID or permit numbers were SCXK-2012-017.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nan Cheng
    • 1
    • 2
  • Yongkun Zhao
    • 1
    • 4
  • Qiuxue Han
    • 1
    • 6
  • Weijiao Zhang
    • 1
  • Ji Xi
    • 3
  • Yongle Yu
    • 3
  • Hualei Wang
    • 1
    • 4
  • Guohua Li
    • 1
    • 5
  • Yuwei Gao
    • 1
    • 4
  • Songtao Yang
    • 1
    • 4
  • Weiquan Liu
    • 3
    • 7
  • Xianzhu Xia
    • 1
    • 4
  1. 1.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military VeterinaryAcademy of Military Medical ScienceChangchunChina
  2. 2.College of Veterinary MedicineChina Agricultural UniversityBeijingChina
  3. 3.College of Biological SciencesChina Agricultural UniversityBeijingChina
  4. 4.Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Disease and ZoonosisYangzhouChina
  5. 5.College of Animal Science and TechnologyShihezi UniversityShiheziChina
  6. 6.College of Veterinary MedicineNortheast Agricultural UniversityHarbinChina
  7. 7.State Key Laboratory of Agro-Biotechnology, Department of Biochemistry and Molecular Biology, College of Biological SciencesChina Agricultural UniversityBeijingChina

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