Genomic characterisation of Cuiaba and Charleville viruses: arboviruses (family Rhabdoviridae, genus Sripuvirus) infecting reptiles and amphibians
- 77 Downloads
Viruses in the family Rhabdoviridae are ecologically very diverse, infecting mammals, birds, reptiles, fish, plants and a wide range of other terrestrial and aquatic invertebrates. The genus Sripuvirus currently comprises five viruses that appear to circulate in transmission cycles involving reptiles and sandflies. Here, we report an analysis of the complete coding sequences of Cuiaba virus (CUIV), isolated from an amphibian (Bufo marinus) collected in Brazil, and Charleville virus (CHVV), isolated from sandflies (Phlebotomus sp.) and lizards (Gehyra australis), collected in Australia. CUIV and CHVV cluster phylogenetically with the sripuviruses in maximum likelihood trees generated from complete L protein (RdRp) sequences. They also share with sripuviruses unique features in genome organisation, including an additional gene (U1) between the matrix protein (M) gene and glycoprotein (G) gene, and an alternative long open reading frame near the start of the G ORF that encodes a predicted transmembrane protein. In view of their phylogenetic relationships, similar genome organisations and similar ecological characteristics, we propose the assignment of CUIV and CHVV as novel members of the genus Sripuvirus.
KeywordsRhabdovirus Sprivivirus Cuiaba virus Charleville virus Genome architecture
Work at UTMB was supported by Grant R24 AI120942 (National Institute of Allergy and Infectious Diseases) and contract HHSN272004/D04 from the National Institutes of Health.
PJW, NV and RBT conceived and planned experiments. NV, SGW and DM performed experiments. PJW and NV analysed data. PJW, NV and RBT wrote the manuscript. All authors reviewed and approved the manuscript.
- 6.Karabatsos N (ed) (1985) International catalogue of arboviruses including certain other viruses of vertebrates. American Society for Tropical Medicine and Hygiene, San AntonioGoogle Scholar
- 8.McAllister J, Gauci PJ, Mitchell IR, Boyle DB, Bulach DM, Weir RP, Melville LF, Davis SS, Gubala AJ (2014) Genomic characterisation of Almpiwar virus, Harrison Dam virus and Walkabout Creek virus; three novel rhabdoviruses from northern Australia. Virol Rep 3:1–17Google Scholar
- 23.Spiropoulou CF, Nichol ST (1993) A small highly basic protein is encoded in overlapping frame within the P gene of vesicular stomatitis virus. J Virol 67:3103–3110Google Scholar
- 24.Peluso RW, Richardson JC, Talon J, Lock M (1996) Identification of a set of proteins (C’ and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase. Virology 218:335–342CrossRefGoogle Scholar