Virus Genes

, Volume 54, Issue 2, pp 280–289 | Cite as

A local strain of Paprika mild mottle virus breaks L3 resistance in peppers and is accelerated in Tomato brown rugose fruit virus-infected Tm-22-resistant tomatoes

  • Neta Luria
  • Elisheva Smith
  • Noa Sela
  • Oded Lachman
  • Ilana Bekelman
  • Amnon Koren
  • Aviv Dombrovsky
Article
First Online:
Received:
Accepted:

Abstract

During October 2014, unfamiliar mild mosaic and mottling symptoms were identified on leaves of pepper (Capsicum chinense cv. Habanero) seedlings grown in the Arava valley in Israel 2–3 weeks post planting. Symptomatic plants were tested positive by ELISA using laboratory-produced antisera for tobamovirus species. Typical tobamovirus rod-shaped morphology was observed by transmission electron microscopy (TEM) analysis of purified virion preparation that was used for mechanical inoculation of laboratory test plants for the completion of Koch’s postulates. The complete viral genome was sequenced from small interfering RNA purified from symptomatic pepper leaves and fruits by next-generation sequencing (NGS) using Illumina MiSeq platform. The contigs generated by the assembly covered 80% of the viral genome. RT-PCR amplification and Sanger sequencing were employed in order to validate the sequence generated by NGS technology. The nucleotide sequence of the complete viral genome was 99% identical to the complete genome of Paprika mild mottle virus isolate from Japan (PaMMV-J), and the deduced amino acid sequence was 99% identical to PaMMV-J protein. Amplicons from seed RNA showed 100% identity to the viral isolate from the collected symptomatic pepper plants. Partial host range analysis revealed a slow development of systemic infection in inoculated tomato plants (Lycopersicon esculentum). Interestingly, double inoculation of susceptible wild-type tomato plants and Tm-22-resistant tomato plants with the PaMMV-IL and Tomato brown rugose fruit virus (ToBRFV) resulted in accelerated viral expression in the plants.

Keywords

Tobamovirus Virus transmission 
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Notes

Acknowledgements

We would like to acknowledge Dr. Nadav Elad from the Electron Microscopy Unit, Departments of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel for the TEM analysis. This work was supported by the Chief Scientist, Israel Ministry of Agriculture, Management of Tm-22 Tobamo breaker in tomato plants, initiative project number 261–1159. Contribution number 575/17 from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.

References

  1. 1.
    A.T.B. Rast, Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 44, 617–622 (1979)Google Scholar
  2. 2.
    I.W. Boukema, Euphytica 29, 433–439 (1980)CrossRefGoogle Scholar
  3. 3.
    I. Tobias, A.T.B. Rast, D. Maat, Eur. J. Plant Pathol. 88, 257–268 (1982)Google Scholar
  4. 4.
    C. Wetter, M. Conti, D. Altschuh, R. Tabillion, M. Van Regenmortel, Phytopathology 74, 405–410 (1984)CrossRefGoogle Scholar
  5. 5.
    A.A. Brunt, K. Crabtree, M. Dallwitz, A. Gibbs, L. Watson, Viruses of plants. Descriptions and lists from the VIDE database (Cab International, Wallingford, 1996)Google Scholar
  6. 6.
    I. Garcia-Luque, M. Ferrero, J. Rodríguez, E. Alonso, A. De La Cruz, A. Sanz, C. Vaquero, M. Serra, J. Diaz-Ruiz, Arch. Virol. 131, 75–88 (1993)CrossRefPubMedGoogle Scholar
  7. 7.
    C.D. Dardick, Z. Taraporewala, B. Lu, J.N. Culver, Mol. Plant Microbe Interact. 12, 247–251 (1999)CrossRefGoogle Scholar
  8. 8.
    A. De La Cruz, L. Lopez, F. Tenllado, J. Diaz-Ruiz, A. Sanz, C. Vaquero, M. Serra, I. Garcia-Luque, Mol. Plant Microbe Interact. 10, 107–113 (1997)CrossRefPubMedGoogle Scholar
  9. 9.
    P. Gilardi, I. Garcia-Luque, M. Serra, Mol. Plant Microbe Interact. 11, 1253–1257 (1998)CrossRefGoogle Scholar
  10. 10.
    P. Gilardi, I. Garcia-Luque, M. Serra, J. Gen. Virol. 85, 2077–2085 (2004)CrossRefPubMedGoogle Scholar
  11. 11.
    M.R. del Pino, A. Moreno, M.G. de Lacoba, S. Castillo-Lluva, P. Gilardi, M. Serra, I. Garcia-Luque, Arch. Virol. 148, 2115–2135 (2003)CrossRefGoogle Scholar
  12. 12.
    H. Hamada, S. Takeuchi, Y. Morita, H. Sawada, A. Kiba, Y. Hikichi, J. Gen. Plant Pathol. 69, 199–204 (2003)Google Scholar
  13. 13.
    K. Matsumoto, H. Sawada, K. Matsumoto, H. Hamada, E. Yoshimoto, T. Ito, S. Takeuchi, S. Tsuda, K. Suzuki, K. Kobayashi, Arch. Virol. 153, 645 (2008)CrossRefPubMedGoogle Scholar
  14. 14.
    H. Sawada, S. Takeuchi, H. Hamada, A. Kiba, M. Matsumoto, Y. Hikichi, J. Jpn. Soc. Hortic. Sci. 73, 552–557 (2004)CrossRefGoogle Scholar
  15. 15.
    K. Gebre-Selassie, G. Marchoux, J. Phytopathol. 131, 275–289 (1991)CrossRefGoogle Scholar
  16. 16.
    H. Mizumoto, K. Kimura, A. Kiba, Y. Hikichi, Virus Res. 153, 205–211 (2010)CrossRefPubMedGoogle Scholar
  17. 17.
    E. Stoimenova, A. Yordanova, Biotechnol Biotec Equip 19, 30–35 (2005)CrossRefGoogle Scholar
  18. 18.
    N. Luria, E. Smith, V. Reingold, I. Bekelman, M. Lapidot, I. Levin, N. Elad, Y. Tam, N. Sela, A. Abu-Ras, N. Ezra, A. Haberman, L. Yitzhak, O. Lachman, A. Dombrovsky, PLoS ONE 12, e0170429 (2017)CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    A. Dombrovsky, R. Sapkota, O. Lachman, M. Pearlsman, Y. Antignus, Plant. Pathol. 62, 450–459 (2013)CrossRefGoogle Scholar
  20. 20.
    U.K. Laemmli, Nature 227, 680–685 (1970)CrossRefPubMedGoogle Scholar
  21. 21.
    M.F. Clark, M.J. Adams, J. Gen. Virol. 34, 475–483 (1977)CrossRefPubMedGoogle Scholar
  22. 22.
    J. Cohen, M. Zeidan, A. Rosner, A. Gera, Phytopathology 90, 340–344 (2000)CrossRefPubMedGoogle Scholar
  23. 23.
    A. Rosner, I. Ginzburg, M. Bar-Joseph, J. Gen. Virol. 64(Pt 8), 1757–1763 (1983)CrossRefPubMedGoogle Scholar
  24. 24.
    A. Dombrovsky, M. Pearlsman, O. Lachman, Y. Antignus, Phytoparasitica 37, 477–483 (2009)CrossRefGoogle Scholar
  25. 25.
    N. Sela, N. Luria, A. Dombrovsky, J. Virol. 86, 7721 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    D.T. Jones, W.R. Taylor, J.M. Thornton, Bioinformatics 8, 275–282 (1992)CrossRefGoogle Scholar
  27. 27.
    K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar, Mol. Biol. Evol. 30, 2725–2729 (2013)CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    J.S. Tung, C.A. Knight, Virol 48, 574–581 (1972)CrossRefGoogle Scholar
  29. 29.
    H. Hamada, S. Takeuchi, K. Akinori, S. Tsuda, Y. Hikichi, T. Okuno, J. Gen. Plant Pathol. 68, 155–162 (2002)CrossRefGoogle Scholar
  30. 30.
    L. Velasco, D. Janssen, L. Ruiz-Garcia, E. Segundo, I.M. Cuadrado, J. Virol. Methods 106, 135–140 (2002)CrossRefPubMedGoogle Scholar
  31. 31.
    Y. Antignus, O. Lachman, M. Pearlsman, L. Maslenin, A. Rosner, Plant Dis. 92, 1033–1037 (2008)CrossRefGoogle Scholar
  32. 32.
    S. Tsuda, M. Kirita, Y. Watanabe, Mol. Plant Microbe Interact. 11, 327–331 (1998)CrossRefPubMedGoogle Scholar
  33. 33.
    H. Hamada, R. Tomita, Y. Iwadate, K. Kobayashi, I. Munemura, S. Takeuchi, Y. Hikichi, K. Suzuki, Virus Genes 34, 205–214 (2007)CrossRefPubMedGoogle Scholar
  34. 34.
    V. Pallas, J.A. García, J. Gen. Virol. 92, 2691–2705 (2011)CrossRefPubMedGoogle Scholar
  35. 35.
    N. Salem, A. Mansour, M. Ciuffo, B. Falk, M. Turina, Arch. Virol. 161, 503–506 (2016)CrossRefPubMedGoogle Scholar
  36. 36.
    R. Li, S. Gao, Z. Fei, K.-S. Ling, Genome Announc. 1, e00794-00713 (2013)Google Scholar
  37. 37.
    C.G. Webster, E.N. Rosskopf, L. Lucas, H.C. Mellinger, S. Adkins, Plant Health Prog. 15, 151–152 (2014)Google Scholar
  38. 38.
    A. Dombrovsky, L.T. Tran-Nguyen, R.A. Jones, Annu. Rev. Phytopathol. 55, 231–256 (2017)CrossRefPubMedGoogle Scholar
  39. 39.
    D. Altschuh, A.M. Lesk, A.C. Bloomer, A. Klug, J. Mol. Biol. 193, 693–707 (1987)CrossRefPubMedGoogle Scholar
  40. 40.
    H. Hamada, S. Takeuchi, A. Kiba, S. Tsuda, K. Suzuki, Y. Hikichi, T. Okuno, J. Gen. Plant Pathol. 71, 90–94 (2005)CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Plant PathologyARO The Volcani CenterBet DaganIsrael
  2. 2.Hishtil Nurseries NehalimMoshav NehalimIsrael

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