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Comparative analysis of in vitro proliferative, migratory and pro-angiogenic potentials of bovine fetal mesenchymal stem cells derived from bone marrow and adipose tissue

  • M. Jervis
  • O. Huaman
  • B. Cahuascanco
  • J. Bahamonde
  • J. Cortez
  • J. I. Arias
  • C. G. Torres
  • O. A. PeraltaEmail author
Original Article

Abstract

Mesenchymal stem cells (MSCs) are found in virtually all tissues, where they self-renew and differentiate into multiple cell types. Cumulative data indicate that MSCs secrete paracrine factors that may play key roles in the treatment of various acute and chronic pathological conditions in diverse animal species including cattle. The aim of the present study was to compare the potentials for proliferation, migration and pro-angiogenesis of bovine fetal BM-MSCs and AT-MSCs under in vitro conditions. Growth curves and population doubling time (PDT) were determined for BM-MSCs and AT-MSCs in order to compare in vitro cell proliferation potentials. The ability of BM-MSCs and AT-MSCs to migrate was evaluated by scratch plate and transwell migration assays. The pro-angiogenic potential of conditioned medium from BM-MSCs and AT-MSCs was compared using an endothelial cell (EC) tubule formation assay. BM-MSCs displayed higher proliferation curves and doubled their populations in fewer days compared to AT-MSCs. No significant differences were detected in the number of migrant cells between BM-MSCs and AT-MSCs; however, a higher migration value was detected for BM-MSCs compared to fibroblasts (FBs), and a higher number of migrant cells were attracted by DMEM supplemented with 5% fetal bovine serum (FBS) compared to stromal cell-derived factor-1 (SDF-1). More tubules of ECs were formed after exposure to concentrated conditioned medium from AT-MSCs compared to BM-MSCs, FBs or DMEM controls. Despite common mesodermal origin, BM-MSCs display higher proliferative capacity and lower pro-angiogenic potential compared to AT-MSCs; however, both cell types possess similar migratory ability.

Keywords

MSCs Cell proliferation Cell migration Angiogenesis 

Notes

Acknowledgements

This study was supported by grants ID15I10129 from the Scientific and Technological Development Support Fund (FONDEF) and 1161251 from the National Commission for Scientific and Technology Research (FONDECYT) from the Ministry of Education, Government of Chile.

Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Compliance with ethical standards

The authors declare that they have no competing interests. This article does not contain any studies with animals performed by any of the authors. All procedures have been approved by the Bioethical Committees of FONDEF and FONDECYT.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Animal Production Science, Faculty of Veterinary SciencesUniversity of ChileSantiagoChile
  2. 2.Department of Biomedical Sciences and PathobiologyVirginia-Maryland Regional College of Veterinary MedicineBlacksburgUSA
  3. 3.Institute of Pharmacology and Morphophysiology, Faculty of Veterinary SciencesAustral University of ChileValdiviaChile
  4. 4.Department of Clinical Science, Faculty of Veterinary SciencesUniversity of ChileSantiagoChile

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