Veterinary Research Communications

, Volume 43, Issue 2, pp 123–129 | Cite as

Quantification of EGFR family in canine mammary ductal carcinomas in situ: implications on the histological graduation

  • Emerson Soares Veloso
  • Ivy Nayra Nascimento Gonçalves
  • Jaqueline Amorim Arantes
  • Rafael Vitor Santos de Abreu
  • Geovanni Dantas Cassali
  • Enio FerreiraEmail author
Original Article


The epithelial growth factor receptors are transmembrane proteins with an important role in the neoplastic progression of tumors, and in this context, DCIS is an important phase in the progression of canine mammary tumors. Studies on the molecular profile and its relationship to a progression of canine mammary tumors are important to improve the treatment of patients and for a better understanding of canine mammary carcinogenesis. The aim of this study was to determine, by immunohistochemistry, the relation between the expression of EGFR, ErbB-2, ErbB-3, and ErbB-4 in 52 canine mammary gland DCIS with high and low histological grade. A positive correlation between histological grade and expression of membrane ErbB-2 and cytoplasmic ErbB-4 was observed. Increased ErbB-4 membrane expression was correlated with increased ErbB-3 expression in low and high-grade DCIS. Our data suggest that increased expression of ErbB-2 and ErbB-4 may be related to more aggressive DCIS and probabily involved with canine mammary neoplastic progression.


Cancer Dog Immunohistochemistry Histological grade EGFR 



The financial support for this study was provided by Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.

Compliance with ethical standards

Conflict of interest

The authors have no competing interests.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Emerson Soares Veloso
    • 1
  • Ivy Nayra Nascimento Gonçalves
    • 1
  • Jaqueline Amorim Arantes
    • 1
  • Rafael Vitor Santos de Abreu
    • 1
  • Geovanni Dantas Cassali
    • 1
  • Enio Ferreira
    • 1
    Email author
  1. 1.Departamento de Patologia Geral, Instituto de Ciências BiológicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil

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