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CD205-positive, Sepharose-induced peritoneal exudate cells: a new resource for DC research in the chicken

  • Christine N. Vuong
  • Wen-Ko Chou
  • Luc R. BerghmanEmail author
Original Article

Abstract

Dendritic cells (DC) are important antigen-presenting cells and are among the least characterized immune cells in the chicken. In order to obtain chicken DC, current protocols require isolation of bone marrow myeloid progenitor cells and induction of DC differentiation with supplemental cytokines or negative selection of splenic cell preparations. Chicken peritoneal exudate cells (PEC) have traditionally been a source of various immune cells for ex vivo studies, primarily to investigate heterophils and macrophages. In this study, we observe the presence of CD205+ PEC populations, a marker of DC, as an additional resource to isolate and study chicken primary DCs. A panel of monoclonal antibodies was developed against the chicken CD205 DC marker and used to isolate CD205+ DC from the PEC population using magnetic bead cell sorting. This study reports the development of new anti-CD205 monoclonal antibodies as a reagent for chicken DC research, as well as PEC as a potential source of CD205+ DC for ex vivo studies in the chicken.

Keywords

Dendritic cell Peritoneal exudate cell CD205 DEC205 Chicken 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Christine N. Vuong
    • 1
  • Wen-Ko Chou
    • 1
  • Luc R. Berghman
    • 1
    • 2
    Email author
  1. 1.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Department of Veterinary PathobiologyTexas A&M UniversityCollege StationUSA

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