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Veterinary Research Communications

, Volume 43, Issue 2, pp 105–114 | Cite as

Genetic association of polymorphisms in bovine TLR2 and TLR4 genes with Mycobacterium avium subspecies paratuberculosis infection in Indian cattle population

  • Satish Kumar
  • Subodh Kumar
  • Ran Vir SinghEmail author
  • Anuj Chauhan
  • Amit Kumar
  • Sourabh Sulabh
  • Jaya Bharati
  • Shoor Vir Singh
Original Article

Abstract

Toll like receptors (TLRs) are pattern recognition molecules involved in cellular recognition of Mycobacterium avium subspecies paratuberculosis (MAP), the infectious agent causing Paratuberculosis (PTB), a notified disease of domestic and wild ruminants. The present study was undertaken to investigate the presence of single nucleotide polymorphisms (SNPs) in TLR2 and TLR4 gene and to evaluate association of these SNPs with occurrence of PTB in Indian cattle. A total of 213 cattle, were subjected to multiple diagnostic tests viz. Johnin PPD, ELISA test (Indigenous and Parachek kit method), fecal microscopy and fecal culture for detection of MAP infection. Based on screening results 51 animals each were assigned to case and control population. Two SNPs viz. rs55617172, rs41830058 in TLR2 gene and two SNPs viz. rs8193046, rs8193060 in TLR4 gene and were genotyped by PCR-RFLP method. All SNPs were found to be polymorphic except rs41830058 in the case-control population. Both SNPs in TLR4 gene but none in TLR2 genes were significantly associated with the occurrence of PTB in our population. The genotypes in SNP rs8193046 and SNP rs8193060 were significantly (P < 0.01) different in case-control population. These findings suggest that SNPs rs8193046 and rs8193060 are likely a potential marker against MAP infection and a selection programme eliminating AG genotype for rs8193046 and CT genotype for rs8193060 might be beneficial in conferring resistance to MAP infection in Indian cattle population.

Keywords

Paratuberculosis SNPs TLR2 TLR4 Immune response Resistance 

Notes

Acknowledgements

We thank Director, Indian Veterinary Research Institute, Izatnagar, India for funding this work.

Compliance with ethical standards

Conflict of interest

The authors do not have any conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Satish Kumar
    • 1
    • 2
  • Subodh Kumar
    • 1
  • Ran Vir Singh
    • 1
    Email author
  • Anuj Chauhan
    • 1
  • Amit Kumar
    • 1
  • Sourabh Sulabh
    • 1
  • Jaya Bharati
    • 2
  • Shoor Vir Singh
    • 3
  1. 1.Division of Animal Genetics and BreedingICAR- Indian Veterinary Research InstituteBareillyIndia
  2. 2.ICAR-National Research Centre on PigGuwahatiIndia
  3. 3.Animal Health DivisionICAR- Central Institute for Research on GoatsMakhdoomIndia

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