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Veterinary Research Communications

, Volume 43, Issue 2, pp 67–76 | Cite as

Unique genetic features of canine adenovirus type 1 (CAdV-1) infecting red foxes (Vulpes vulpes) in northern Norway and arctic foxes (Vulpes lagopus) in Svalbard

  • Andrea BalboniEmail author
  • Morten Tryland
  • Torill Mørk
  • Siw T. Killengreen
  • Eva Fuglei
  • Mara Battilani
Original Article
  • 122 Downloads

Abstract

Canine adenovirus type 1 (CAdV-1) is the aetiological agent of infectious canine hepatitis (ICH) in domestic dogs (Canis familiaris). In spite of the widespread use of vaccination, CAdV-1 continues to circulate in the dog population. Although a high number of serological screenings have indicated that CAdV-1 is widespread in fox species, little is known about the potential role of foxes as reservoirs of CAdV-1. Furthermore, very little data exist on the molecular features of this virus in foxes. To add to existing knowledge on CAdV-1 circulating in wild carnivores, tissue samples from CAdV-seropositive red foxes (Vulpes vulpes, n = 10) from the northern mainland of Norway and arctic foxes (Vulpes lagopus, n = 10) from the Svalbard archipelago, Norway, were investigated using a molecular approach to detect CAdV-1 DNA and important structural and non-structural genes of the detected viruses were sequenced and analysed. Amplicons characteristic for CAdV-1 were amplified from 14 out of 20 foxes (7 red foxes and 7 arctic foxes) and spleen and lymph node tissues resulted optimal targets for the viral DNA detection. The nucleotide sequences showed unique features that distinguished the viruses detected in this study from the CAdV-1 to date identified in wild carnivores and dogs. Greater attention should be given to genetically different CAdV-1 circulating in wild carnivores that may be transferred to dogs, potentially causing disease and reducing the effectiveness of available vaccines.

Keywords

Canine adenovirus Fox Genetic characterization Mastadenovirus Norway Wildlife 

Notes

Acknowledgements

We thank all the trappers and hunters that provided the fox samples. Funding for sampling the fox tissues were provided from the Norwegian Polar Institute and financed by the Norwegian Environment Agency to Climate –ecological Observatory for Arctic Tundra (COAT). We also acknowledge Dr. Javier Sánchez Romano for help with the tissue samples and DNA extraction.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Veterinary Medical SciencesAlma Mater Studiorum-University of BolognaOzzano Emilia (BO)Italy
  2. 2.Arctic Infection Biology, Department of Arctic and Marine BiologyUiT Arctic University of NorwayTromsøNorway
  3. 3.Norwegian Polar Institute, Fram CentreTromsøNorway
  4. 4.Section of PathologyNorwegian Veterinary InstituteTromsøNorway
  5. 5.Department of EducationUiT Arctic University of NorwayTromsøNorway

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