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Plant Ecology

, Volume 220, Issue 1, pp 29–39 | Cite as

Root-associated fungi increase male fitness, while high simulated herbivory decreases indirect defenses in Croton lachnostachyus plants

  • Mariana Pereyra
  • Gabriel GrilliEmail author
  • Leonardo Galetto
Article

Abstract

Plants interact with a diverse array of organisms below and above ground; some interactions with ants allow plants to be protected against herbivorous insects, influencing their growth or reproduction. In addition, indirect plant defenses—such as those mediated by extrafloral nectaries (EFNs)—could be affected by plant root symbionts. However, it is not clear how the suppression of root symbionts might affect extrafloral nectar (EFN) production and plant reproductive output. We made an experimental study with a shrub species with EFNs. Firstly, we tested if root-associated fungi (i.e., comparing plants with and without fungicide) increased the production of pollen (male function) and EFN (volume, nectar concentration, and total sugar content) in Croton lachnostachyus. Subsequently, we implemented a second experiment on the same plants, adding different levels of simulated herbivory (none, low, high) to assess the combined effects of root-associated fungi and herbivory. While we found high levels of mycorrhizal colonization, we found no signs of pathogenic fungi and negligible values of dark septate endophytes in roots so we attributed our results mostly to arbuscular mycorrhizal fungi (AMF). The first experiment showed that plants without the fungicide treatment increased pollen production and secreted a lower mean volume of EFN with higher concentration of dissolved soluble solids. In the second experiment, high levels of simulated herbivory showed a diminution on EFN variables; also, we detected a lower shoot dry mass on plants with low levels of herbivory and no interactions with AMF. Our results suggest complex ecological responses related to the root-associated fungal community and simulated herbivory.

Keywords

Extrafloral nectar (EFN) Pollen Root-associated fungi Shoot dry mass Simulated herbivory Arbuscular mycorrhizal fungi (AMF) 

Notes

Acknowledgements

We thank two anonymous reviewers and Marina Omacini for useful comments and suggestions on a previous version of this manuscript. Also, we thank Romina Fernández for improving the English. This work was funded by FONCYT, the National University of Cordoba (SECyT—UNC), and CONICET. MP is a postdoctoral fellow in FONCYT. GG and LG are researchers from CONICET. Also, LG and MP are professors at the Universidad Nacional de Córdoba.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Mariana Pereyra
    • 1
  • Gabriel Grilli
    • 1
    Email author
  • Leonardo Galetto
    • 1
    • 2
  1. 1.Instituto Multidisciplinario de Biología Vegetal, FCEFyN, (CONICET-Universidad Nacional de Córdoba)CórdobaArgentina
  2. 2.Departamento de Diversidad Biológica y Ecología, Facultad de Ciencias ExactasFísicas y Naturales Universidad Nacional de CórdobaCórdobaArgentina

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