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The effects of oxytocin on penile tissues in experimental priapism model in rats

  • Engin Kolukcu
  • Sahin Kilic
  • Bekir Suha Parlaktas
  • Fikret Erdemir
  • Velid Unsal
  • Dogan Atılgan
  • Nihat Uluocak
Urology - Original Paper

Abstract

Purpose

This study aimed to demonstrate the effects of oxytocin on penile tissues in ischemia–reperfusion injury developed after priapism.

Methods

Forty Wistar Albino strain male rats were divided into four groups. The control group (n = 10) was not intervened. In Group 2, a rat model of priapism was constructed and maintained for 1 h. In Group 3, reperfusion was ensured for 30 min following priapism. Rats in Group 4 rats were given oxytocin 30 min before the induction of reperfusion following priapism. All rats were penectomized, and adequate amounts of blood sample were drawn. Inflammation, vasocongestion, desquamation, and edema in penile tissue were scored between 0 and 3 points (0: normal, 1: mild, 2: moderate, 3: severe) to evaluate the severity of tissue damage. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the levels of malondialdehyde (MDA), and nitric oxide (NO) in blood samples were determined spectrophotometrically.

Results

In histopathological examination, statistically significant positive changes were detected in vasocongestion, inflammation, desquamation, and edema scores in Group 4 than in Group 2 and Group 3 (p < 0.001). Biochemical test results revealed that NO levels were significantly lower in Group 4 than in Group 3 (p < 0.001). Serum GSH-Px activities in Group 4 significantly increased when compared with the other groups 2 and 3 (p = 0.002, p = 0.001, respectively). There was no statistical difference among the groups regarding SOD activities and MDA levels (p > 0.05).

Conclusions

Oxytocin protected against priapism-induced ischemia–reperfusion injury developed in cavernosal tissue as observed based on histopathological and biochemical evidence. Although this is an experimental study, oxytocin can be thought as an alternative drug in the treatment of priapism.

Keywords

Ischemia–reperfusion injury Oxytocin Penile tissue Priapism Rat model 

Notes

Compliance with ethical standards

Conflict of interest

The authors report no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of UrologyTokat State HospitalTokatTurkey
  2. 2.Department of UrologyFethiye State HospitalFethiyeTurkey
  3. 3.Department of Urology, Faculty of MedicineGaziosmanpasa UniversityTokatTurkey
  4. 4.Mardin Artuklu University High School of Health and Central Research LaboratoryMardinTurkey

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