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Comparison of ruminal digestibility of Origanum onites L. leaves in dairy buffalo and cows

Abstract

This experiment evaluated the ruminal digestibility of Turkish oregano leaves in dairy buffalo and cows. Ruminally cannulated, multiparous Brown Swiss cows (n = 3) and water buffalo (Bubalus bubalis; n = 3) were used in the experiment. The ad libitum basal diet was balanced to NRC requirements for a dry, nonpregnant multiparous dairy cow (680 kg) and consuming 12.8 kg of DM/day. Air-dried, ground, weighed oregano leaves were inserted in the rumen of all animals before the morning feeding within heat-sealed nylon bags (4 replicates per treatment and time point). After incubation (4, 8, 24, 48, and 72 h), dry matter (DM), crude protein (CP), aNDFom, and ADFom concentrations were determined and compared to initial leaf chemical composition. The means for in situ disappearance (ISD, %) of DM, aNDFom, and ADFom did not differ between the species, but ISD of CP was greater in buffalo than the cows (P = 0.05) after 72 h incubation. The lag time of kinetic degradation curves, the potentially degradable fraction (b), hourly degradation rate (c), and undegradable fraction were similar between species. The immediately degraded fraction (a) and effective digestibility (ED) of CP were greater in buffalo than the cows (P = 0.04), but the a fraction and ED of other nutrients showed no differences between the species. In conclusion, CP of oregano leaves are degraded more rapidly in the rumen of dairy buffalo than cows, primarily due to a large difference in the rapidly soluble CP fraction in the buffalo.

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Abbreviations

a :

Immediately degraded fraction

ADL:

Acid detergent lignin

ADFom:

Acid detergent fiber expressed exclusive of residual ash

aNDFom:

Neutral detergent fiber assayed with a heat-stable amylase and expressed exclusive of residual ash

ATP:

Adenosine triphosphate

b :

Potentially degradable fraction

BW:

Body weight

c :

Hourly degradation rate

CNCPS:

Cornell net carbohydrate and protein system

CP:

Crude protein

DM:

Dry matter

DMI:

Dry matter intake

ED:

Effective digestibility

ISD:

In situ disappearance

ME:

Metabolizable energy

NEL :

Net energy lactation

NFC:

Non-fiber carbohydrates

OM:

Organic matter

SEM:

Standard error of means

TMR:

Total mixed ration

UF:

Undegradable fraction

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Acknowledgments

The authors thank Dr. Mustafa KARGIOGLU (Department of Molecular Biology and Genetics, Afyon Kocatepe University, Turkey) for identifying the species of the plants (Origanum onites L.) and the staff of Afyon Kocatepe University Animal Teaching & Research Center (Afyonkarahisar, Turkey) for their assistance with this study.

Funding

This study was financially supported by the Research Projects Commission of Afyon Kocatepe University (grant no. 16. KARIYER.119).

Author information

Correspondence to Eyup Eren Gultepe.

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The authors declare that they have no conflict of interest.

Research involving animals

The experiment was conducted under the approval of Afyon Kocatepe University Animal Care and Use Committee (CASE No. 49533702/143).

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Gultepe, E.E., Uyarlar, C., Cetingul, I.S. et al. Comparison of ruminal digestibility of Origanum onites L. leaves in dairy buffalo and cows. Trop Anim Health Prod (2020). https://doi.org/10.1007/s11250-020-02233-6

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Keywords

  • Oregano
  • In situ
  • Mediterranean buffalo