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Hematologic, lipid profile, immunity, and antioxidant status of growing rabbits fed black seed as natural antioxidants

  • Yassmine El-GindyEmail author
  • Hassan Zeweil
  • Soliman Zahran
  • Mohamed Abd El-Rahman
  • Fawzi Eisa
Regular Articles
  • 7 Downloads

Abstract

It is known that using antibiotics by way of growth promoters has harmful side effects on both animals and consumer health due to cross-resistance among pathogens and residues in meat. Using antibiotic to promote growth has been banned in the most countries for this reason; so, we must search for feed additives alternative in animal production. One of them is the black seed (Nigella sativa). Fifty-four growing V-line unsexed rabbits, 4 weeks of age, with an average weight of 776.7 g were randomly allocated to one of three treatments (n = 18): rabbits fed the basal diet (control), rabbits supplemented with either 300 or 600 mg of Nigella sativa seed (NSS)/kg diet. At 12 weeks of age, NSS supplementation significantly improved final body weight, weight gain, feed conversion ratio (FCR), and performance index with significantly reduced feed consumption. Addition of 300 and 600 mg NSS/kg diet resulted in a significant increase in the number of RBCs and WBCs as compared to basal diet group. Compared to controls, NSS supplementation significantly stimulated the IgG and IgM immune responses of rabbits, significantly reduced serum total lipids, triglycerides, and low-density lipoprotein, and significantly increased the high-density lipoprotein concentration. All NSS treatments significantly increased the observed blood total antioxidant capacity and significantly decreased the malondialdehyde values, compared to the basal diet group. In conclusion, the results displayed that addition of NSS in rabbit diets improved productive performance, blood lipid profile, immunity, and antioxidant status, and supplementation with 600 mg/kg NSS was more effective than 300 mg/kg NSS seed supplementation.

Keywords

Rabbits Black seed Immunity Blood constituents 

Notes

Compliance with ethical standards

The manuscript does not contain clinical studies or patient data.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abbasnezhad, A., Hayatdavoudi, P., Niazmand, S. and Mahmoudabady, M., 2015. The effects of hydroalcoholic extract of Nigella sativa seed on oxidative stress in hippocampus of STZ-induced diabetic rats. Avicenna Journal of Phytomedicine, 5,333–340.PubMedPubMedCentralGoogle Scholar
  2. Ali, B. H. and Blunden, G., 2003. Pharmacological and toxicological properties of Nigella sativa. Phytotherapy Research, 17 (4), 299–305CrossRefGoogle Scholar
  3. Association of Official Analytical Chemists (AOAC) 2000. Official Method of Analysis, 17th edition. ACOC, Arlington.Google Scholar
  4. Burits, M. and F. Bucar. 2000. Antioxidant activity of Nigella sativa essential oil. Phototherapy Research, 14, 323-328.Google Scholar
  5. Cheeke, P.R., Patton, N.M., Lukefahr, S.D. and McNitt, J.I., 1987. Rabbit Production, 6th edition. The Interstate Printers & Publishers, Inc. Danville.Google Scholar
  6. Dhama, K., Tiwari, R., Khan, R.U., Chakraborty, S., Gopi, M., Karthik, K., Saminathan, M., Desingu, P. A. and Sunkara, L.T. 2014. Growth promoters and novel feed additives improving poultry production and health, bioactive principles and beneficial applications: The trends and advances-a review. International Journal of pharmacology, 10, 129–159.CrossRefGoogle Scholar
  7. El-Beshbishy, H.A., Singab, A.N.B., Sinkkonen, J. and Pihlaja, K., 2006. Hypolipidemic and antioxidant effects of Morus alba L. (Egyptian mulberry) root bark fractions supplementation in cholesterol-fed rats. Life Science, 78, 2724–2733.CrossRefGoogle Scholar
  8. El-Dakhakhny, M., Barakat, M., El-Halim, M.A. and Aly, S.M., 2000. Effects of Nigella sativa oil on gastric secretion and ethanol induced ulcer in rats. Journal of Ethnopharmacology, 72, 299–304.CrossRefGoogle Scholar
  9. Ghasemi, H.A., Kasani, N. and Taherpour, K., 2014. Effects of black cumin seed (Nigella sativa L.), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers. Livestock Science, 164, 128–134.CrossRefGoogle Scholar
  10. Hepler, O.E., 1966. Manual of Clinical Laboratory Methods. (4th ed). Charles C Thomas publisher, Illinois.Google Scholar
  11. Jamroz, D. and Kamel, C., 2002. Plant extracts enhance broiler performance in non-ruminant nutrition: Antimicrobial agents and plant extracts on immunity, health and performance. Journal of Animal Science, 80, 41–46.Google Scholar
  12. Kaur, R., Arora, S. and Singh, B., 2008. Antioxidant activity of the Phenol rich fractions of Chukrasia tabularis A.Juss. leaves. Bioresource Technology, 99, 7692–98CrossRefGoogle Scholar
  13. Khan, T.A. and Zafar, F., 2005. Haematological study in response to various doses of estrogen in broiler production. International Journal of Poultry Science, 40(10), 748–751.Google Scholar
  14. Lutterodt, H., Luther, M., Slavin, M., Yin, J.J., Parry, J. and Gao, J. M., 2010. Fatty acid profile, thymoquinone content, oxidative stability, and antioxidant properties of cold-pressed black cumin seed oils. LWT- Food Science and Technology, 43, 1409–1413.CrossRefGoogle Scholar
  15. Majdalawieh, A.F. and Fayyad, M.W., 2015. Immunomodulatory and anti-inflammatory action of Nigella sativa and thymoquinone: A comprehensive review. International Immunopharmacology, 28, 295–304.CrossRefGoogle Scholar
  16. National Research Council (NRC) 1977. Nutrient requirements of domestic animals. 2nd revised edition. National Academy of Science. Washington.Google Scholar
  17. Natt, M. P. and Herrick, C. A., 1952. A new blood diluent for counting erythrocytes and leucocytes of the chicken. Poultry Science, 31, 735–738.CrossRefGoogle Scholar
  18. Nelson, N.A., Lakshmanan, N. and Lamont, S. J., 1995. Sheep red blood cell and Brucella abortus antibody responses in chickens selected for multitrait immunocompetence. Poultry Science, 74, 1603–1609.CrossRefGoogle Scholar
  19. North, M.O., 1981. Commercial Chicken production. Manual 2nd Edition Library of Congress AVI Publishing Company, INC, USA.Google Scholar
  20. Paarakh, P.M., 2010. Nigella sativa Linn. – A comprehensive review. Indian Journal of Natural Products and Resources, 1, 409–429.Google Scholar
  21. Padhye, S., Banerjee, S., Ahmad, A., Mohammad, R. and Sarkar, F.H., 2008. From here to eternity-the secret of pharaohs: Therapeutic potential of black cumin seeds and beyond. Journal of Cancer Therapy, 6, 495–510.Google Scholar
  22. Pourghassem-Gargari, B., Ebrahimzadeh-Atary, V., Rafraf, M. and Gorbani, A. 2009. Effect of dietary supplementation with Nigela sativa L. on serum lipid profile, lipid peroxidation and antioxidant defense system in hyperlipidemic rabbits. Journal of Medicinal Plants Research, 3 (10), 815–821.Google Scholar
  23. SPSS Statistical Packages for the Social Sciences 2001. Statistical software for windows version 11.0 Microsoft. SPSS ®, Chicago, IL, USA.Google Scholar
  24. Talati, R., Baker, W.L., Pabilonia, M.S., White, C.M. and Coleman, C.I., 2009. The effects of barley-derived soluble fiber on serum lipids. The Annals of Family Medicine, 7 (2), 157–163CrossRefGoogle Scholar
  25. Tiwari, R. and Dhama, K., 2014. Antibiotic resistance: A frightening health dilemma. American Journal of Pharmacology and Toxicology, 9, 174–176.CrossRefGoogle Scholar
  26. Wegmann, T.G. and Smithies, O., 1966. A simple hemagglutination system requiring small amount of red cells and antibodies. Transfusion, 6, 67–73.CrossRefGoogle Scholar
  27. Williams, C. S., Mann, M. and DuBois, R.N., 1999. The role of cyclooxygenases in inflammation, cancer and development. Oncogene, 18, 7908–7916.CrossRefGoogle Scholar
  28. Zaoui, A., Cherrah, Y., Alaoui, K., Mahassine, N., Amarouch, H. and Hassar, M., 2002. Effects of Nigella sativa fixed oil on blood homeostasis in rat. Journal of Ethnopharmacology, 79 (1), 23–26.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Animal and Fish Production, Faculty of Agriculture (Saba Basha)University of AlexandriaAlexandriaEgypt
  2. 2.Department of Agriculture SciencesHigher Institute of Science and TechnologyMsehel-TrpoleLibya

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