This study investigated the effects of streptozotocin (STZ) and S-allyl-L-cysteine (SAC) on motility, plasma membrane integrity, and mitochondrial activity of the boar sperm. STZ (0, 10, 50, and 100 μM) and SAC (0, 1, 5, 25, and 100 μM) were treated alone and co-treated in the fresh boar semen. The motility, plasma membrane integrity, and mitochondrial activity of sperm were analyzed at 3, 6, and 9 h after incubation. Boar semen was collected using the gloved-hand method from ten crossbred male pigs, and age of experimental ten male pigs is 24~27 months. The sperm plasma membrane integrity was analyzed using Live/Dead sperm kit. Mitochondrial activity was analyzed using rhodamine 123 and PI double-staining method. Additionally, sperm motility was evaluated according to standard method. Sperm motility, plasma membrane integrity, and mitochondrial activity were decreased in an STZ concentration-dependent manner (P < 0.05) and also were decreased by 10 μM STZ in all incubation times (P < 0.05). The motility, plasma membrane integrity, and mitochondrial activity of the sperm were increased at 5 μM SAC treatment, whereas it was decreased at 100 μM treatment. In addition, sperm motility, plasma membrane integrity, and mitochondrial activity were increased when co-treated with 50 μM STZ and 5 μM SAC group at 9 h after incubation (P < 0.05). Based on our results, STZ has a deleterious effect on sperm characteristics, and SAC can protect sperm motility, viability, and function of the sperm exposed to STZ.
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This work was supported by 2017 Research Grant from Kangwon National University (no. 520170021).
SL and B-KY designed the experimental study and drafted the manuscript. S-HL analyzed the results and discussed the manuscript. A-SL performed the sperm plasma membrane integrity, motility, and mitochondrial activity assay. All authors read and approved the final manuscript.
Compliance with ethical standards
All procedures involving the use of animal experiments were approved by the Kangwon National University Institutional Animal Care and Use Committee (KIACUC-09-0139).
The authors declare that they have no competing interests.
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