Tropical Animal Health and Production

, Volume 51, Issue 8, pp 2595–2601 | Cite as

Effect of fat supplementation on physiological and reproductive performance of Holstein Friesian bulls during summer

  • Mahboob Ahmed Butt
  • Jalees Ahmed Bhatti
  • Anjum Khalique
  • Muhammad Qamer ShahidEmail author
Regular Articles


The objective of current study was to determine the effect of fat supplement on physiological and reproductive performance of Holstein Friesian bulls during subtropical summer in Pakistan. Eighteen bulls were randomly divided into 3 treatment groups: (1) CTL, basal diet without fat supplementation; (2) FS100, basal diet with 100 g fat supplementation (Energizer-RP-10®, IFFCO, Johor, Malaysia); (3) FS200, basal diet with 200 g fat supplementation. Basal diet consisted of 50% green fodder (corn silage), 25% wheat straw, and 25% concentrate on dry matter basis. Diets were offered for 14 weeks from May to August 2016. The average daily temperature-humidity index ranged from 85 to 88 for the experimental period. The results indicated that there was no difference in dry matter intake, water intake, rectal temperature, pulse rate, and respiration rate among the treatment groups. Fat supplementation did not influence semen traits including sperm motility, progressive motility, amplitude of lateral head displacement, live-to-dead ratio, normal acrosomal ridge, plasma membrane integrity, and DNA integrity. The interaction of season with fat revealed that FA200 significantly increased post thaw semen motility and progressive motility during hot humid summer (P < 0.05). Analysis of blood metabolites showed that blood urea nitrogen was higher in FS200 compared with CTL and FS100 groups (P < 0.05). There was no effect of fat supplementation on blood testosterone level. Glucose showed quadratic trend in response to fat supplementation. It could be concluded that addition of fat only improved motility and progressive motility of sperms during hot humid summer.


Fat supplementation Heat stress Semen traits Holstein Friesian bulls 



The authors thank Mr. Imran (Lecturer, Department of Biochemistry, Ravi Campus, UVAS) for his assistance in chemical analysis. The authors also acknowledge the contribution of the laboratory staff (Dr. Mirza Salman Saleem and Dr. Shazia Mansoor), technicians, and bull handlers at semen production unit.

Compliance with ethical standards

Statement of animal rights

The experimental procedures were approved by Ethical Review Committee, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Livestock ProductionUniversity of Veterinary and Animal SciencesLahorePakistan
  2. 2.Department of Animal SciencesCollege of Veterinary and Animal SciencesJhangPakistan
  3. 3.Department of Animal NutritionUniversity of Veterinary and Animal SciencesLahorePakistan

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